Meadows, John C., Lancaster, Theresa C., Buttrick, Graham J., Sochaj, Alicja, Messin, Liam J., Mora-Santos, Maria, Hardwick, Kevin G. and Millar, Jonathan B. A. (2017) Identification of a Sgo2-dependent but Mad2-independent pathway controlling anaphase onset in fission yeast. Cell Reports, 18 (6). pp. 1422-1433. doi:10.1016/j.celrep.2017.01.032 ISSN 2211-1247.

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Abstract

The onset of anaphase is triggered by activation of the anaphase promoting complex/cyclosome (APC/C), following silencing of the spindle assembly checkpoint (SAC). APC/C triggers ubiquitination of Securin and Cyclin B, which leads to loss of sister chromatid cohesion and inactivation of Cyclin B/Cdk1, respectively. This promotes relocalisation of Aurora B kinase, and other components of the chromosome passenger complex (CPC), from centromeres to the spindle midzone. In fission yeast this is mediated by Clp1 phosphatase-dependent interaction of CPC with Klp9/MKLP2 (kinesin-6). When this interaction is disrupted, kinetochores bi-orient normally but APC/C activation is delayed via a mechanism that requires Sgo2 and some (Bub1, Mph1/Mps1, Mad3), but not all (Mad1 and Mad2), components of the SAC and the first, but not second, KEN box in Mad3. These data indicate that interaction of CPC with Klp9 terminates a Sgo2-dependent, but Mad2-independent, APC/C-inhibitory pathway that is distinct from the canonical SAC.

Item Type:	Journal Article
Alternative Title:
Subjects:	Q Science > QH Natural history
Divisions:	Other > Institute of Advanced Study Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Spindle (Cell division), Chromosomes, Centromere
Journal or Publication Title:	Cell Reports
Publisher:	Elsevier Inc.
ISSN:	2211-1247
Official Date:	7 February 2017
Dates:	Date Event 7 February 2017 Published 15 January 2017 Accepted
Volume:	18
Number:	6
Page Range:	pp. 1422-1433
DOI:	10.1016/j.celrep.2017.01.032
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	30 January 2017
Date of first compliant Open Access:	30 March 2017
Funder:	University of Warwick Institute of Advanced Study Scholarship, Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England)
Grant number:	Program grant MR/K001000/1 (MRC), Program grant 083610 (Wellcome Trust (London, England))
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID UNSPECIFIED University of Warwick Institute of Advanced Study Scholarship http://dx.doi.org/10.13039/501100000741 MR/K001000/1 [MRC] Medical Research Council http://dx.doi.org/10.13039/501100000265 083610 Wellcome Trust http://dx.doi.org/10.13039/100010269

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