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Identification of a Sgo2-dependent but Mad2-independent pathway controlling anaphase onset in fission yeast
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Meadows, John C., Lancaster, Theresa C., Buttrick, Graham J., Sochaj, Alicja, Messin, Liam J., Mora-Santos, Maria, Hardwick, Kevin G. and Millar, Jonathan B. A. (2017) Identification of a Sgo2-dependent but Mad2-independent pathway controlling anaphase onset in fission yeast. Cell Reports, 18 (6). pp. 1422-1433. doi:10.1016/j.celrep.2017.01.032 ISSN 2211-1247.
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Official URL: http://dx.doi.org/10.1016/j.celrep.2017.01.032
Abstract
The onset of anaphase is triggered by activation of the anaphase promoting complex/cyclosome (APC/C), following silencing of the spindle assembly checkpoint (SAC). APC/C triggers ubiquitination of Securin and Cyclin B, which leads to loss of sister chromatid cohesion and inactivation of Cyclin B/Cdk1, respectively. This promotes relocalisation of Aurora B kinase, and other components of the chromosome passenger complex (CPC), from centromeres to the spindle midzone. In fission yeast this is mediated by Clp1 phosphatase-dependent interaction of CPC with Klp9/MKLP2 (kinesin-6). When this interaction is disrupted, kinetochores bi-orient normally but APC/C activation is delayed via a mechanism that requires Sgo2 and some (Bub1, Mph1/Mps1, Mad3), but not all (Mad1 and Mad2), components of the SAC and the first, but not second, KEN box in Mad3. These data indicate that interaction of CPC with Klp9 terminates a Sgo2-dependent, but Mad2-independent, APC/C-inhibitory pathway that is distinct from the canonical SAC.
Item Type: | Journal Article | ||||||||||||
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Alternative Title: | |||||||||||||
Subjects: | Q Science > QH Natural history | ||||||||||||
Divisions: | Other > Institute of Advanced Study Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Spindle (Cell division), Chromosomes, Centromere | ||||||||||||
Journal or Publication Title: | Cell Reports | ||||||||||||
Publisher: | Elsevier Inc. | ||||||||||||
ISSN: | 2211-1247 | ||||||||||||
Official Date: | 7 February 2017 | ||||||||||||
Dates: |
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Volume: | 18 | ||||||||||||
Number: | 6 | ||||||||||||
Page Range: | pp. 1422-1433 | ||||||||||||
DOI: | 10.1016/j.celrep.2017.01.032 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 30 January 2017 | ||||||||||||
Date of first compliant Open Access: | 30 March 2017 | ||||||||||||
Funder: | University of Warwick Institute of Advanced Study Scholarship, Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England) | ||||||||||||
Grant number: | Program grant MR/K001000/1 (MRC), Program grant 083610 (Wellcome Trust (London, England)) | ||||||||||||
RIOXX Funder/Project Grant: |
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