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Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H
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Casillo, Angela, Ståhle, Jonas, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Pieretti, Giuseppina, Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parrilli, Michelangelo, Widmalm, Göran, Tutino, Maria L. and Corsaro, Maria M. (2017) Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H. Antonie van Leeuwenhoek, 110 (11). pp. 1377-1387. doi:10.1007/s10482-017-0834-6 ISSN 0003-6072.
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Official URL: http://dx.doi.org/10.1007/s10482-017-0834-6
Abstract
Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: →4)-β-d-GlcpNAcA-(1 →3)-β-d-QuipNAc4NAc-(1 →3)-β-d-GalpNAc-(1 →. The 3D model, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 °C, this molecule displays only a low ice recrystallization inhibition activity.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Cold adaptation, Extracellular space, Microbial polysaccharides | ||||||||
Journal or Publication Title: | Antonie van Leeuwenhoek | ||||||||
Publisher: | Springer | ||||||||
ISSN: | 0003-6072 | ||||||||
Official Date: | November 2017 | ||||||||
Dates: |
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Volume: | 110 | ||||||||
Number: | 11 | ||||||||
Page Range: | pp. 1377-1387 | ||||||||
DOI: | 10.1007/s10482-017-0834-6 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 20 February 2017 | ||||||||
Date of first compliant Open Access: | 4 February 2018 | ||||||||
Funder: | Statens råd för byggnadsforskning (Sweden) [Swedish Research Council], Knut och Alice Wallenbergs Stiftelse (Sweden) | ||||||||
Open Access Version: |
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