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Self-establishing communities enable cooperative metabolite exchange in a eukaryote
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Campbell, Kate, Vowinckel, Jakob, Mülleder, Michael, Malmsheimer, Silke, Lawrence, Nicola, Calvani, Enrica, Miller-Fleming, Leonor, Alam, Mohammad T., Christen, Stefan, Keller, Markus A. and Ralser, Markus (2015) Self-establishing communities enable cooperative metabolite exchange in a eukaryote. eLife, 4 . e09943.
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Official URL: http://dx.doi.org/10.7554/eLife.09943.001
Abstract
Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function. Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nucleotide metabolism regularly fail to compensate for each other's deficiencies upon co-culturing, a situation which implied the absence of growth-relevant metabolite exchange interactions. Contrastingly, we find that yeast colonies maintain a rich exometabolome and that cells prefer the uptake of extracellular metabolites over self-synthesis, indicators of ongoing metabolite exchange. We conceived a system that circumvents co-culturing and begins with a self-supporting cell that grows autonomously into a heterogeneous community, only able to survive by exchanging histidine, leucine, uracil, and methionine. Compensating for the progressive loss of prototrophy, self-establishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner, maintaining a wild-type like exometabolome, growth parameters, and cell viability. Yeast, as a eukaryotic model, thus possesses extensive capacity for growth-relevant metabolite exchange and readily cooperates in metabolism within progressively establishing communities.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QK Botany Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Metabolites, Saccharomyces cerevisiae, Yeast, Eukaryotic cells | ||||||||
| Journal or Publication Title: | eLife | ||||||||
| Publisher: | eLife Sciences Publications Ltd. | ||||||||
| ISSN: | 2050-084X | ||||||||
| Official Date: | 26 October 2015 | ||||||||
| Dates: |
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| Date of first compliant deposit: | 8 March 2017 | ||||||||
| Volume: | 4 | ||||||||
| Article Number: | e09943 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Wellcome Trust (London, England), European Research Council (ERC), Isaac Newton Trust, Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF) | ||||||||
| Grant number: | RG 093735/Z/10/Z (Wellcome Trust), StG 260809 (ERC), RG 68998 (Isaac Newton Trust), J3341 (FWF) |
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