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Cellular reactions to long-term volatile organic compound (VOC) exposures
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Gostner, Johanna M., Zeisler, Johannes, Alam, Mohammad T., Gruber, Peter, Fuchs, Dietmar, Becker, Kathrin, Neubert, Kerstin, Kleinhappl, Markus, Martini, Stefan and Überall, Florian (2016) Cellular reactions to long-term volatile organic compound (VOC) exposures. Scientific Reports, 6 . 37842. ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/srep37842
Abstract
Investigations of cellular processes initiated by volatile organic compounds (VOCs) are limited when modelling realistic long-term exposure scenarios at low concentrations. Exposure to indoor VOCs is associated with a range of adverse effects, but data on molecular changes at regulatory threshold limits are lacking. Activity analysis of VOC in vitro can be a valuable complement to inhalation toxicological evaluations. We developed an exposure platform that generates a stable VOC atmosphere and allows the exposure of cells for longer periods. Using formaldehyde as a model analyte, air-liquid interface cultured A549 lung epithelial cells were exposed to critical concentrations of 0.1 and 0.5 ppm for 3 days. Owing to the lack of known exposure biomarkers, we applied a genome-wide transcriptional analysis to investigate cellular responses at these sublethal concentrations. We demonstrate a minor overlap of differentially expressed transcripts for both treatment concentrations, which can be further analyzed for their use as exposure biomarkers. Moreover, distinct expression patterns emerge for 0.1 and 0.5 ppm formaldehyde exposure, which is reflected in significant enrichment of distinct biological processes. More specifically, metabolism of specific compound classes, lipid biosynthesis and lung-associated functions are affected by lower exposure levels and processes affecting proliferation and apoptosis dominate the higher exposure levels.
| Item Type: | Journal Article | ||||||||
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| Subjects: | T Technology > TD Environmental technology. Sanitary engineering | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Volatile organic compounds -- Health aspects | ||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 2045-2322 | ||||||||
| Official Date: | 1 December 2016 | ||||||||
| Dates: |
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| Volume: | 6 | ||||||||
| Article Number: | 37842 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Date of first compliant deposit: | 8 March 2017 | ||||||||
| Date of first compliant Open Access: | 8 March 2017 | ||||||||
| Funder: | Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF), Österreichische Forschungsförderungsgesellschaft (FFG) | ||||||||
| Grant number: | MUI-START project 2014-05-023 (FWF), FFG834169 (FFG) |
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