The Library
The metabolic background is a global player in Saccharomyces gene expression epistasis
Tools
Tools
Tools
Alam, Mohammad T., Zelezniak, Aleksej, Mülleder, Michael, Shliaha, Pavel, Schwarz, Roland, Capuano, Floriana, Vowinckel, Jakob, Radmaneshfar, Elahe, Krüger, Antje, Calvani, Enrica, Michel, Steve, Börno, Stefan, Christen, Stefan, Patil, Kiran Raosaheb, Timmermann, Bernd, Lilley, Kathryn S. and Ralser, Markus (2016) The metabolic background is a global player in Saccharomyces gene expression epistasis. Nature microbiology, 1 . 15030.
Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1038/nmicrobiol.2015.30
Abstract
The regulation of gene expression in response to nutrient availability is fundamental to the genotype–phenotype relationship. The metabolic–genetic make-up of the cell, as reflected in auxotrophy, is hence likely to be a determinant of gene expression. Here, we address the importance of the metabolic–genetic background by monitoring transcriptome, proteome and metabolome in a repertoire of 16 Saccharomyces cerevisiae laboratory backgrounds, combinatorially perturbed in histidine, leucine, methionine and uracil biosynthesis. The metabolic background affected up to 85% of the coding genome. Suggesting widespread confounding, these transcriptional changes show, on average, 83% overlap between unrelated auxotrophs and 35% with previously published transcriptomes generated for non-metabolic gene knockouts. Background-dependent gene expression correlated with metabolic flux and acted, predominantly through masking or suppression, on 88% of transcriptional interactions epistatically. As a consequence, the deletion of the same metabolic gene in a different background could provoke an entirely different transcriptional response. Propagating to the proteome and scaling up at the metabolome, metabolic background dependencies reveal the prevalence of metabolism-dependent epistasis at all regulatory levels. Urging a fundamental change of the prevailing laboratory practice of using auxotrophs and nutrient supplemented media, these results reveal epistatic intertwining of metabolism with gene expression on the genomic scale.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
||||||||
| Journal or Publication Title: | Nature microbiology | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 2058-5276 | ||||||||
| Official Date: | 1 February 2016 | ||||||||
| Dates: |
|
||||||||
| Volume: | 1 | ||||||||
| Article Number: | 15030 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Adapted As: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
