Jannat, Shopan, Mou, Haipeng, Zhang, Lili, Zhang, Changtong, Ma, Weiwei, Walsh, John A., Hu, Zhongyuan, Yang, Jinghua and Zhang, Mingfang (2017) Eukaryotic translation initiation factor 2B-beta (eIF2B β), a new class of plant virus resistance gene. The Plant Journal, 90 (5). pp. 929-940. doi:10.1111/tpj.13519 ISSN 0960-7412.

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Abstract

Recessive resistances to plant viruses in the Potyvirus genus have been found to be based on mutations in the plant eukaryotic translation initiation factors, eIF4E and eIF4G or their isoforms. Here we report that natural, monogenic recessive resistance to the potyvirus Turnip mosaic virus (TuMV) has been found in a number of mustard (Brassica juncea) accessions. Bulked segregant analysis and sequencing of resistant and susceptible plant lines indicated the resistance is controlled by a single recessive gene, recessive TuMV resistance 03 (retr03), an allele of the eukaryotic translation initiation factor 2B-beta (eIF2Bβ). Silencing of eIF2Bβ in a TuMV-susceptible mustard plant line and expression of eIF2Bβ from a TuMV-susceptible line in a TuMV-resistant mustard plant line confirmed the new resistance mechanism. A functional copy of a specific allele of eIF2Bβ is required for efficient TuMV infection. eIF2Bβ represents a new class of virus resistance gene conferring resistance to any pathogen. eIF2B acts as a guanine nucleotide exchange factor (GEF) for its GTP-binding protein partner eIF2 via interaction with eIF2·GTP at an early step in translation initiation. Further genotyping indicated that a single non-synonymous substitution (A120G) in the N-terminal region of eIF2Bβ was responsible for the TuMV resistance. A reproducible marker has been developed, facilitating marker-assisted selection for TuMV resistance in B. juncea. Our findings provide a new target for seeking natural resistance to potyviruses and new opportunities for the control of potyviruses using genome editing techniques targeted on eIF2Bβ.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Brassica juncea, Potyvirus diseases, Turnip mosaic virus
Journal or Publication Title:	The Plant Journal
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	0960-7412
Official Date:	June 2017
Dates:	Date Event June 2017 Published 28 February 2017 Available 15 February 2017 Accepted 8 July 2016 Submitted
Volume:	90
Number:	5
Page Range:	pp. 929-940
DOI:	10.1111/tpj.13519
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Date of first compliant deposit:	9 March 2017
Date of first compliant Open Access:	28 February 2018
Funder:	Zhōngguó zhèjiāng shěng zìrán kēxué jījīn (China) [Zhejiang Provincial Natural Science Foundation of China] (ZPNSFC), China. Nong ye bu [Ministry of Agriculture]
Grant number:	2015C32046 (ZPNSFC), 09162130135252 (China. Nong ye bu)
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