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Physiological induction of transient receptor potential canonical proteins, calcium entry channels, in human myometrium: Influence of pregnancy, labor, and interleukin-1 beta
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UNSPECIFIED (2004) Physiological induction of transient receptor potential canonical proteins, calcium entry channels, in human myometrium: Influence of pregnancy, labor, and interleukin-1 beta. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 89 (3). pp. 1291-1300. doi:10.1210/jc.2003-031428 ISSN 0021-972X.
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Official URL: http://dx.doi.org/10.1210/jc.2003-031428
Abstract
This study investigated gestational regulation of transient receptor potential canonical (TrpC) proteins, putative calcium entry channels in human myometrium, and the potential modulation of TrpC expression by IL-1beta, a cytokine implicated in labor. Total RNA and proteins were isolated from myometrial biopsies obtained from NP women, pregnant women at term not in labor (TNL), or term active labor ( TAL) and from primary cultured human myometrial smooth muscle cells incubated with IL-1beta or IL-1beta with or without nimesulide. Semiquantitative RT-PCR demonstrated significant upregulation of TrpC1 in TAL and TNL ( P less than or equal to 0.01) and TrpC6 ( P less than or equal to 0.01) and TrpC7 ( P less than or equal to 0.05) in TAL samples. TrpC3 and TrpC4 mRNA expression was unaffected. Western blot demonstrated significant up-regulation of TrpC1 in TAL and TNL ( P less than or equal to 0.05) and TrpC3 ( P less than or equal to 0.01), TrpC4 ( P less than or equal to 0.05), and TrpC6 ( P less than or equal to 0.01) in TAL samples. IL-1beta did not alter TrpC1, 3, 4, 6, or 7 mRNA expression; but IL-1beta exclusively up-regulated TrpC3 protein expression ( P less than or equal to 0.05). TrpC3 up-regulation was unaffected by cyclooxygenase blockade. These data demonstrate physiological regulation of TrpC mRNA and protein and suggest an important role for TrpC proteins in human myometrium during labor.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RC Internal medicine | ||||
Journal or Publication Title: | JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | ||||
Publisher: | ENDOCRINE SOC | ||||
ISSN: | 0021-972X | ||||
Official Date: | March 2004 | ||||
Dates: |
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Volume: | 89 | ||||
Number: | 3 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 1291-1300 | ||||
DOI: | 10.1210/jc.2003-031428 | ||||
Publication Status: | Published |
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