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Carbohydrate-protein interactions at interfaces: comparison of the binding of Ricinus communis lectin to two series of synthetic glycolipids using surface plasmon resonance studies
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UNSPECIFIED (2003) Carbohydrate-protein interactions at interfaces: comparison of the binding of Ricinus communis lectin to two series of synthetic glycolipids using surface plasmon resonance studies. ORGANIC & BIOMOLECULAR CHEMISTRY, 1 (23). pp. 4148-4159. doi:10.1039/b306784j ISSN 1477-0520.
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Official URL: http://dx.doi.org/10.1039/b306784j
Abstract
Two Glactosyl lipids and the related C-galactosyl lipids have been synthesised and their binding to RCA(120) plant lectin was compared with a second series of thiolactosylethoxyalkanes. The interactions were measured quantitatively in real time by surface plasmon resonance (BIAcore) at a range of concentrations and temperatures from 5 to 30degreesC. The C-galactosyl lipid (1,3-dimethyl-5-[beta-D-galactopyranosyl]-5-(4-octadecyloxybenzyl)pyrimidine-2,4,6-trione) bound much more weakly with a K-A = 8.86 x 10(5) than the corresponding Glactosyl lipid (1,3-dimethyl-5-[beta-D-galactopyranosyl-(1-->4)-beta-D-glucopyranosyl]-5-(4-octadecyloxybenzyl)pyrimidine-2,4,6-trione) (K-A = 2.31 x 10(7)). The influence of the linker region of the two different series of lactosyl lipids was clearly demonstrated by the differences in the binding to RCA(120) lectin. The changes in kinetic values and in the enthalpic and entropic contribution to the free energy of binding reflected the importance of the linker and the hydrocarbon anchor holding the synthetic glycolipids in the neomembrane.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | ORGANIC & BIOMOLECULAR CHEMISTRY | ||||
Publisher: | ROYAL SOC CHEMISTRY | ||||
ISSN: | 1477-0520 | ||||
Official Date: | 2003 | ||||
Dates: |
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Volume: | 1 | ||||
Number: | 23 | ||||
Number of Pages: | 12 | ||||
Page Range: | pp. 4148-4159 | ||||
DOI: | 10.1039/b306784j | ||||
Publication Status: | Published |
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