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Integrating the genetic and physical chromosome maps of Brassica oleracea var. alboglabra
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Armstrong, S. J., Howell, E. C., Fransz, G. H., Kearsey, M. J., King, G. J., Kop, E., Ryder, Carol D., Teakle, Graham R. and Vicente, Joana G. (2000) Integrating the genetic and physical chromosome maps of Brassica oleracea var. alboglabra. In: King, G. J., (ed.) Proceedings of the Third International Symposium on Brassicas and Twelfth Crucifer Genetics Workshop : Wellesbourne, United Kingdom, 5-9 September, 2000. Acta horticulturae (539). Leuven : ISHS, pp. 77-82. ISBN 9789066059238
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Official URL: https://www.worldcat.org/title/proceedings-of-the-...
Abstract
Linking genetic maps constructed with molecular markers to the corresponding physical maps is an important goal in the investigation of crops. The relationship between these maps is reviewed, and we illustrate our approach by the analysis of the short arm of chromosome 4 of the model dicotyledonous plant, Arabidopsis thaliana L. We show that it is possible to produce a detailed molecular cytogenetic map by placing multicopy and unique sequences in relation to euchromatic and heterochromatic segments. This map correlates well with the molecular physical map (sequence map) but, as expected, shows differences from the genetic map.
For Brassica oleracea L., as a first step we have produced a partial karyotype based on the combination of chromosome size, arm ratios and the location of repeated 45S rDNA, 5S rDNA and pericentromeric heterochromatin sequences by fluorescence in situ hybridisation (FISH). We are currently using BACs, cosmids and RFLPs, with known genetic map positions, as FISH probes to identify additional linkage group associated markers for the chromosomes. The construction of a definitive karyotype, with all linkage groups assigned to our cytogenetic map is approaching completion.
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Series Name: | Acta horticulturae | ||||||
Publisher: | ISHS | ||||||
Place of Publication: | Leuven | ||||||
ISBN: | 9789066059238 | ||||||
Book Title: | Proceedings of the Third International Symposium on Brassicas and Twelfth Crucifer Genetics Workshop : Wellesbourne, United Kingdom, 5-9 September, 2000 | ||||||
Editor: | King, G. J. | ||||||
Official Date: | 2000 | ||||||
Dates: |
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Number: | 539 | ||||||
Number of Pages: | 143 | ||||||
Page Range: | pp. 77-82 | ||||||
Status: | Not Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 2 October 2017 | ||||||
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