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Tailored emulsion-templated porous polymer scaffolds for iPSC-derived human neural precursor cell culture
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Murphy, Ashley R., Ghobrial, Irene, Jamshidi, Pegah, Laslett, Andrew, O'Brien, Carmel M. and Cameron, Neil R. (2017) Tailored emulsion-templated porous polymer scaffolds for iPSC-derived human neural precursor cell culture. Polymer Chemistry, 8 (43). 6617-6627 . doi:10.1039/C7PY01375B ISSN 1759-9954.
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Official URL: http://dx.doi.org/10.1039/C7PY01375B
Abstract
The work here describes the synthesis of tailor-made, porous, polymeric materials with elastic moduli in the range associated with mammalian brain tissue (0.1–24 kPa). Three new emulsion-templated porous polymer materials (polyHIPEs) were synthesised by thiol–ene photopolymerisation from hexanediol diacrylate (HDDA) and polyethylene glycol diacrylate (PEGDA) crosslinkers and compared with a previously reported material prepared from trimethylolpropane triacrylate (TMPTA). The materials were found to have an average pore diameter of 30–63 μm and a porosity of 77% and above. PEGDA crosslinked materials at 80 and 85% porosity, when swollen in PBS at 37 °C, were found to have an elastic modulus of 18 and 9.0 kPa respectively. PEGDA crosslinked materials were also found to have a swelling ratio of 700% in PBS at 37 °C. PEGDA crosslinked materials had improved visible light transmission properties when compared to TMPTA crosslinked materials under a bright field microscope. All materials were shown via hematoxylin and eosin staining to support the infiltration and attachment of induced pluripotent stem cell (iPSC)-derived human neural progenitor cells (hNPCs). HNPCs on all materials were demonstrated in short term 3D cultures to maintain a phenotype consistent with early neural lineage specification via immunohistochemical staining for the intermediate filament protein vimentin.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||||
Journal or Publication Title: | Polymer Chemistry | ||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||
ISSN: | 1759-9954 | ||||||||||
Official Date: | 20 November 2017 | ||||||||||
Dates: |
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Volume: | 8 | ||||||||||
Number: | 43 | ||||||||||
Page Range: | 6617-6627 | ||||||||||
DOI: | 10.1039/C7PY01375B | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 31 October 2017 | ||||||||||
Date of first compliant Open Access: | 6 October 2018 |
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