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Axisymmetric contact problem for a flattened cell : contributions of substrate effect and cell thickness to the determination of viscoelastic properties by using AFM indentation
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Liu, Xianping, Zhu, Xinyao, Wang, Zuobin and Liu, lanjiao (2017) Axisymmetric contact problem for a flattened cell : contributions of substrate effect and cell thickness to the determination of viscoelastic properties by using AFM indentation. Scanning . doi:10.1155/2017/8519539 ISSN 0161-0457.
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WRAP-axisymmetric-contact-flattened-cell-substrate-viscoelastic-Zhu-2017.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1389Kb) | Preview |
Official URL: https://doi.org/10.1155/2017/8519539
Abstract
Nanoindentation technology has proven an effective method to investigate the viscoelastic properties of biological cells. The experimental data obtained by nanoindentation are frequently interpreted by Hertz contact model. However, in order to facilitate the application of Hertz contact model, a mass of studies assume cells have infinite thickness which does not necessarily represent the real situation. In this study, a rigorous contact model based upon linear elasticity is developed for the interpretation of indentation tests of flattened cells which represent a factual morphology. The cell, normally bonded to the petri dish, is initially treated as an elastic layer of finite thickness perfectly fixed to a rigid substrate, and the conic indenter is assumed to be frictionless. The theory of linear elasticity is utilized to solve this contact issue and then the solutions are extended to viscoelastic situation which is regarded as a good indicator for mechanical properties of biological cells. To test the present model, an AFM-based creep test has been conducted on living human hepatocellular carcinoma cell (SMMC-7721 cell) and its fullerenol-treated counterpart. The results indicate that the present model could not only describe very well the creep behavior of SMMC-7721 cells, but can also curb overestimation of the mechanical properties due to substrate effect. Moreover, the present model could identify the difference between the control and treated SMMC-7721 cells in terms of the extracted viscoelastic parameters, suggesting its potential in revealing the biomechanical effects of fullerenol-like drug treatment on cancerous cells.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||
| Library of Congress Subject Headings (LCSH): | Atomic force microscopy, Viscoelasticity, Cells -- Measurement | ||||||
| Journal or Publication Title: | Scanning | ||||||
| Publisher: | Hidawi | ||||||
| ISSN: | 0161-0457 | ||||||
| Official Date: | 20 December 2017 | ||||||
| Dates: |
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| DOI: | 10.1155/2017/8519539 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 1 November 2017 | ||||||
| Date of first compliant Open Access: | 2 November 2017 | ||||||
| Funder: | Horizon 2020 (European Commission) (H2020) | ||||||
| Grant number: | Marie Skłodowska - Curie grant agreement No 644971, FP7 MCA-IRSES (612641), China-EU research programme (S2016G4501) |
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