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The c-terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its Golgi-mediated sorting to the vacuole
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Hadlington, Jane L., Santoro, Aniello, Nuttall, James, Denecke, J. (Jurgen), Ma, Julian K.-C., Vitale, A. (Alessandro) and Frigerio, Lorenzo (2003) The c-terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its Golgi-mediated sorting to the vacuole. Molecular Biology of the Cell, Vol.14 (No.6). pp. 2592-2602. doi:10.1091/mbc.E02-11-0771 ISSN 1059-1524.
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Official URL: http://dx.doi.org/10.1091/mbc.E02-11-0771
Abstract
We have assessed the ability of the plant secretory pathway to handle the expression of complex heterologous proteins by investigating the fate of a hybrid immunoglobulin A/G in tobacco cells. Although plant cells can express large amounts of the antibody, a relevant proportion is normally lost to vacuolar sorting and degradation. Here we show that the synthesis of high amounts of IgA/G does not impose stress on the plant secretory pathway. Plant cells can assemble antibody chains with high efficiency and vacuolar transport occurs only after the assembled immunoglobulins have traveled through the Golgi complex. We prove that vacuolar delivery of IgA/G depends on the presence of a cryptic sorting signal in the tailpiece of the IgA/G heavy chain. We also show that unassembled light chains are efficiently secreted as monomers by the plant secretory pathway.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QK Botany Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Library of Congress Subject Headings (LCSH): | Golgi apparatus, Immunoglobulin A, Immunoglobulin G, Plant vacuoles | ||||
| Journal or Publication Title: | Molecular Biology of the Cell | ||||
| Publisher: | American Society for Cell Biology | ||||
| ISSN: | 1059-1524 | ||||
| Official Date: | June 2003 | ||||
| Dates: |
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| Volume: | Vol.14 | ||||
| Number: | No.6 | ||||
| Page Range: | pp. 2592-2602 | ||||
| DOI: | 10.1091/mbc.E02-11-0771 | ||||
| Status: | Peer Reviewed | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), British Council, Italy. Ministero dell'università e della ricerca scientifica e tecnologica | ||||
| Grant number: | 88/C13441 (BBSRC), ROM/889/99/10 (BC/MURST) |
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