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Mechanisms involved in acquisition of blaNDM genes by IncA/C2 and IncFIIY plasmids
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Wailan, Alexander M., Sidjabat, Hanna E., Yam, Wan Keat, Alikhan, Nabil-Fareed, Petty, Nicola K., Sartor, Anna L., Williamson, Deborah A., Forde, Brian M., Schembri, Mark A., Beatson, Scott A., Paterson, David L., Walsh, Timothy R. and Partridge, Sally R. (2016) Mechanisms involved in acquisition of blaNDM genes by IncA/C2 and IncFIIY plasmids. Antimicrobial Agents and Chemotherapy, 60 (7). pp. 4082-4088. doi:10.1128/AAC.00368-16 ISSN 0066-4804.
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Official URL: http://dx.doi.org/10.1128/AAC.00368-16
Abstract
blaNDM genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility
(Inc) groups. Here we present the complete sequences of four plasmids carrying a blaNDM gene, pKP1-NDM-1, pEC2-
NDM-3, pECL3-NDM-1, and pEC4-NDM-6, from four clinical samples originating from four different patients. Different plasmids
carry segments that align to different parts of the blaNDM region found on Acinetobacter plasmids. pKP1-NDM-1 and
pEC2-NDM-3, from Klebsiella pneumoniae and Escherichia coli, respectively, were identified as type 1 IncA/C2 plasmids with
almost identical backbones. Different regions carrying blaNDM are inserted in different locations in the antibiotic resistance island
known as ARI-A, and ISCR1 may have been involved in the acquisition of blaNDM-3 by pEC2-NDM-3. pECL3-NDM-1 and
pEC4-NDM-6, from Enterobacter cloacae and E. coli, respectively, have similar IncFIIY backbones, but different regions carrying
blaNDM are found in different locations. Tn3-derived inverted-repeat transposable elements (TIME) appear to have been involved
in the acquisition of blaNDM-6 by pEC4-NDM-6 and the rmtC 16S rRNA methylase gene by IncFIIY plasmids. Characterization
of these plasmids further demonstrates that even very closely related plasmids may have acquired blaNDM genes by different
mechanisms. These findings also illustrate the complex relationships between antimicrobial resistance genes, transposable
elements, and plasmids and provide insights into the possible routes for transmission of blaNDM genes among species of the Enterobacteriaceae
family.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Antimicrobial Agents and Chemotherapy | ||||||||
Publisher: | American Society for Microbiology | ||||||||
ISSN: | 0066-4804 | ||||||||
Official Date: | July 2016 | ||||||||
Dates: |
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Volume: | 60 | ||||||||
Number: | 7 | ||||||||
Page Range: | pp. 4082-4088 | ||||||||
DOI: | 10.1128/AAC.00368-16 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 6 December 2017 |
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