The Library
Discrimination effects in MALDI-MS of mixtures of peptides - Analysis of the Proteome
Tools
UNSPECIFIED (2003) Discrimination effects in MALDI-MS of mixtures of peptides - Analysis of the Proteome. AUSTRALIAN JOURNAL OF CHEMISTRY, 56 (5). pp. 369-377. doi:10.1071/CH02155 ISSN 0004-9425.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1071/CH02155
Abstract
Peptide ion suppression in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) can hinder the detection of site-specific post-translational protein modifications. Within a peptide mixture, the presence or absence of a particular peptide can affect the ion intensities of other peptides in the mixture. These effects have been studied using equimolar solutions of target peptides and observation of the increase or decrease in ion intensity of the peptides upon the removal or addition of individual peptides. Gas-phase basicities and hydrophobicity measures have been used to rationalize this behaviour. ZipTips have been used to remove impurities and reduce the number of peptides present at any moment in a solution, a procedure that results in a significant increase in the total percentage of the amino acid coverage of enzymatically digested proteins. The efficacy of this approach was demonstrated using specifically nitrated lysozyme and specifically nitrated myoglobin.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | AUSTRALIAN JOURNAL OF CHEMISTRY | ||||
Publisher: | C S I R O PUBLISHING | ||||
ISSN: | 0004-9425 | ||||
Official Date: | 2003 | ||||
Dates: |
|
||||
Volume: | 56 | ||||
Number: | 5 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 369-377 | ||||
DOI: | 10.1071/CH02155 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |