The Library
Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily
Tools
Crow, Allister, Greene, Nicholas P., Kaplan, Elise and Koronakis, Vassilis (2017) Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily. Proceedings of the National Academy of Sciences of the United States of America, 114 (47). pp. 12572-12577. doi:10.1073/pnas.1712153114 ISSN 0027-8424.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1073/pnas.1712153114
Abstract
MacB is an ABC transporter that collaborates with the MacA adaptor protein and TolC exit duct to drive efflux of antibiotics and enterotoxin STII out of the bacterial cell. Here we present the structure of ATP-bound MacB and reveal precise molecular details of its mechanism. The MacB transmembrane domain lacks a central cavity through which substrates could be passed, but instead conveys conformational changes from one side of the membrane to the other, a process we term mechanotransmission. Comparison of ATP-bound and nucleotide-free states reveals how reversible dimerization of the nucleotide binding domains drives opening and closing of the MacB periplasmic domains via concerted movements of the second transmembrane segment and major coupling helix. We propose that the assembled tripartite pump acts as a molecular bellows to propel substrates through the TolC exit duct, driven by MacB mechanotransmission. Homologs of MacB that do not form tripartite pumps, but share structural features underpinning mechanotransmission, include the LolCDE lipoprotein trafficking complex and FtsEX cell division signaling protein. The MacB architecture serves as the blueprint for understanding the structure and mechanism of an entire ABC transporter superfamily and the many diverse functions it supports.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||||
Publisher: | National Academy of Sciences | ||||||
ISSN: | 0027-8424 | ||||||
Official Date: | 21 November 2017 | ||||||
Dates: |
|
||||||
Volume: | 114 | ||||||
Number: | 47 | ||||||
Page Range: | pp. 12572-12577 | ||||||
DOI: | 10.1073/pnas.1712153114 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |