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Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis
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Yu, Zhiming, Chen, Qiyuan, Chen, Weiwei, Zhang, Xian, Mei, Fengling, Zhang, Pengcheng, Zhao, Mei, Wang, Xiaohong, Shi, Nongnong, Jackson, Stephen D. and Hong, Yiguo (2018) Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis. Journal of Integrative Plant Biology, 60 (5). pp. 376-381. doi:10.1111/jipb.12622 ISSN 1672-9072.
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Official URL: http://dx.doi.org/10.1111/jipb.12622
Abstract
We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms.
Item Type: | Journal Article | ||||||||||||||||||||||||
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Subjects: | Q Science > QK Botany | ||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Arabidopsis -- Genetic engineering, Crossing over (Genetics) | ||||||||||||||||||||||||
Journal or Publication Title: | Journal of Integrative Plant Biology | ||||||||||||||||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||||||||||||||
ISSN: | 1672-9072 | ||||||||||||||||||||||||
Official Date: | May 2018 | ||||||||||||||||||||||||
Dates: |
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Volume: | 60 | ||||||||||||||||||||||||
Number: | 5 | ||||||||||||||||||||||||
Page Range: | pp. 376-381 | ||||||||||||||||||||||||
DOI: | 10.1111/jipb.12622 | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
Date of first compliant deposit: | 10 January 2018 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 11 December 2018 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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