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Determination of boron content using a simple and rapid miniaturized curcumin assay
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Mohan, Thotegowdanapalya C. and Jones, Alexandra M. (2018) Determination of boron content using a simple and rapid miniaturized curcumin assay. Bio-protocol, 7 (2). doi:10.21769/BioProtoc.2703 ISSN 2331-8325.
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Official URL: http://dx.doi.org/10.21769/BioProtoc.2703
Abstract
To determine boron quantity in soil, water and biological samples, several protocols are available. Colorimetric assays are the simplest and cheapest methods which can be used to determine boron concentration. However, published protocols do not give straightforward guidance for beginners to adopt these protocols for routine use in the laboratory. Based on a previously published available procedure, we present a detailed and modified version of a curcumin based colorimetric protocol to determine boron concentration extracted from any sample. Our modified protocol is able to determine up to 0.2 nmole of Boron in a sample volume of 300 µl.
Item Type: | Journal Article | ||||||
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Subjects: | S Agriculture > S Agriculture (General) | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Soils -- Boron content -- Testing -- Methodology, Colorimetric analysis | ||||||
Journal or Publication Title: | Bio-protocol | ||||||
Publisher: | Bio-protocol LLC | ||||||
ISSN: | 2331-8325 | ||||||
Official Date: | 20 January 2018 | ||||||
Dates: |
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Volume: | 7 | ||||||
Number: | 2 | ||||||
DOI: | 10.21769/BioProtoc.2703 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 7 February 2018 | ||||||
Date of first compliant Open Access: | 7 February 2018 | ||||||
RIOXX Funder/Project Grant: |
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