(2010) Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana. Molecular Systems Biology, Vol.6 (No.397). doi:10.1038/msb.2010.53 ISSN 1744-4292.
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Abstract
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)–cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A- and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA;1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK–cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QK Botany |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) |
| Library of Congress Subject Headings (LCSH): | Growth (Plants) -- Molecular aspects, Arabidopsis thaliana -- Growth, Arabidopsis thaliana -- Genetics, Cyclin-dependent kinases, Plant cell cycle |
| Journal or Publication Title: | Molecular Systems Biology |
| Publisher: | Nature Publishing Group |
| ISSN: | 1744-4292 |
| Official Date: | 10 August 2010 |
| Dates: | Date Event 10 August 2010 Published |
| Volume: | Vol.6 |
| Number: | No.397 |
| DOI: | 10.1038/msb.2010.53 |
| Status: | Peer Reviewed |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Funder: | Agentschap voor Innovatie door Wetenschap en Technologie (Belgium) (IWT), Belgium. Politique scientifique fédérale [Belgian State Science Policy Office] (BELSPO), European Commission (EC), National Science Foundation (U.S.) (NSF), Fonds Wetenschappelijk Onderzoek (FWO) |
| Grant number: | GBOU 20193 (IWT), AGRON-OMICS LSHG-CT-2006-037704 (EC), IOS 0744566 (NSF) |
| URI: | https://wrap.warwick.ac.uk/3486/ |
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