Murakami, Keisuke, Bhandari, Harish, Lucas, Emma S., Takeda, Satoru, Gargett, Caroline E., Quenby, Siobhan, Brosens, Jan J. and Tan, Bee K. (2013) Deficiency in clonogenic endometrial mesenchymal stem cells in obese women with reproductive failure – a pilot study. PLoS One, Volume 8 (Number 12). Article Number e82582 . doi:10.1371/journal.pone.0082582 ISSN 1932-6203.
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Abstract
The mechanisms of obesity associated reproductive complications remain poorly understood. Endometrial mesenchymal stem-cells are critical for cyclic renewal and uterine function. Recently, W5C5+ cells, with high clonogenicity, capable of producing endometrial stroma in vivo, have been described. We sought to investigate the abundance and cloning efficiency of W5C5+ and W5C5− endometrial cells in relation to Body Mass Index, age and reproductive outcome.
Design
W5C5+ and W5C5− cells were purified from mid-luteal endometrial biopsies (n = 54) by magnetic bead separation and subjected to in vitro colony-forming assays.
Results
First trimester pregnancy losses were significantly higher in obese subjects (n = 12) compared to overweight (n = 20) and subjects with normal Body Mass Index (n = 22) (P<0.05, P<0.01, respectively). W5C5+ cells (%) were significantly lower in obese subjects compared to subjects with normal Body Mass Index (P<0.05). W5C5+ cloning efficiency was significantly lower in obese subjects compared to overweight and subjects with normal Body Mass Index (P<0.05, respectively). W5C5− cloning efficiency was significantly lower in obese subjects compared to subjects with normal Body Mass Index (P<0.05). Body Mass Index was significantly negatively correlated with W5C5+ cloning efficiency and W5C5− cloning efficiency (P<0.01, respectively), and positively correlated with first trimester loss (P<0.01). We found no significant results with age (P>0.05).
Conclusions
Our observations suggest that the regenerative capacity and plasticity of the endometrium of obese women is suboptimal, which in turn may account for the increased risk of reproductive complications associated with obesity.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > RG Gynecology and obstetrics |
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Obesity in women, Pregnancy -- Complications, Endometrium -- Physiology, Mesenchymal stem cells |
| Journal or Publication Title: | PLoS One |
| Publisher: | Public Library of Science |
| ISSN: | 1932-6203 |
| Official Date: | 2013 |
| Dates: | Date Event 2013 Published |
| Volume: | Volume 8 |
| Number: | Number 12 |
| Article Number: | Article Number e82582 |
| DOI: | 10.1371/journal.pone.0082582 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 26 December 2015 |
| Date of first compliant Open Access: | 26 December 2015 |
| Funder: | University Hospitals Coventry and Warwickshire NHS Trust, Warwick Medical School, National Health and Medical Research Council (Australia) (NHMRC) , Victoria |
| Grant number: | 1042298 (NHMRC) |
| URI: | https://wrap.warwick.ac.uk/59501/ |
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