Windheim, Mark, Höning, Stefan, Leppard, Keith, Butler, Larrisa, Seed, Christina, Ponnambalam, Sreenivasan and Burgert, Hans-Gerhard (2016) Sorting motifs in the cytoplasmic tail of the immunomodulatory E3/49K protein of species D adenoviruses modulate cell surface expression and ectodomain shedding. Journal of Biological Chemistry, 291 . pp. 6796-6812. doi:10.1074/jbc.M115.684787 ISSN 0021-9258.
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Abstract
The E3 transcription unit of human species C adenoviruses (Ads) encodes immunomodulatory proteins that mediate direct protection of infected cells. Recently, we described a novel immunomodulatory function for E3/49K, an E3 protein uniquely expressed by species D Ads. E3/49K of Ad19a/Ad64, a serotype that causes epidemic keratokonjunctivitis, is synthesized as a highly glycosylated type I transmembrane protein that is subsequently cleaved resulting in secretion of its large ectodomain (sec49K). Sec49K binds to CD45 on leukocytes, impairing activation and functions of NK cells and T cells. E3/49K is localized in the Golgi/trans-Golgi-network (TGN), early endosomes and on the plasma membrane, yet the cellular compartment where E3/49K is cleaved and the protease involved remained elusive. Here we show that TGN-localized E3/49K comprises both newly-synthesized and recycled molecules. Full-length E3/49K was not detected in late endosomes/lysosomes but the C-terminal fragment accumulated in this compartment at late times of infection. Inhibitor studies showed that cleavage occurs in a post-TGN compartment and that lysosomotropic agents enhance secretion. Interestingly, the cytoplasmic tail of E3/49K contains two potential sorting motifs, YxxΦ and LL that are important for binding the clathrin adaptor proteins AP-1 and AP-2 in vitro. Surprisingly, mutating the LL motif, either alone or together with YxxΦ, did not prevent proteolytic processing, but increased cell surface expression and secretion. Upon Brefeldin-A treatment cell surface expression was rapidly lost, even for mutants lacking all known endocytosis motifs. Together with immunofluorescence data, we propose a model for intracellular E3/49K transport whereby cleavage takes place on the cell surface by matrix-metalloproteases.
Item Type: | Journal Article |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
Journal or Publication Title: | Journal of Biological Chemistry |
Publisher: | American Society for Biochemistry and Molecular Biology |
ISSN: | 0021-9258 |
Official Date: | 25 March 2016 |
Dates: | Date Event 25 March 2016 Published 3 February 2016 Available 3 February 2016 Accepted 12 August 2015 Submitted |
Volume: | 291 |
Page Range: | pp. 6796-6812 |
DOI: | 10.1074/jbc.M115.684787 |
Status: | Peer Reviewed |
Publication Status: | Published |
Access rights to Published version: | Restricted or Subscription Access |
URI: | https://wrap.warwick.ac.uk/77779/ |
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