2023

Kirkman, Tim, Tan, Suk Fun, Pacheco, Sair Maximo Chavez, Hammer, Alexander, Tosin, Manuela, Coyne, Anthony and Dias, Marcio (2023) Fragment merging strategies with known pyrimidine scaffolds targeting dihydrofolate reductase from Mycobacterium tuberculosis. ChemMedChem . doi:10.1002/cmdc.202300240 ISSN 1860-7179.

Zhang, Songya, Zhang, Lin, Greule, Anja, Tailhades, Julien, Marschall, Edward, Prasongpholchai, Panward, Leng, Daniel J., Zhang, Jingfan, Zhu, Jing, Kaczmarski, Joe A., Schittenhelm, Ralf B., Einsle, Oliver, Jackson, Colin J., Alberti, Fabrizio, Bechthold, Andreas, Zhang, Youming, Tosin, Manuela, Si, Tong and Cryle, Max J. (2023) P450-mediated dehydrotyrosine formation during WS9326 biosynthesis proceeds via dehydrogenation of a specific acylated dipeptide substrate. Acta Pharmaceutica Sinica B . doi:10.1016/j.apsb.2023.03.021 ISSN 2211-3835. (In Press)

2021

Izoré, Thierry, Candace Ho, Y T, Kaczmarski, Joe A, Gavriilidou, Athina, Chow, Ka Ho, Steer, David L, Goode, Robert J A, Schittenhelm, Ralf B, Tailhades, Julien, Tosin, Manuela, Challis, Gregory L., Krenske, Elizabeth H, Ziemert, Nadine, Jackson, Colin J and Cryle, Max J (2021) Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity. Nature Communications, 12 (1). 2511. doi:10.1038/s41467-021-22623-0 ISSN 2041-1723.

Leng, Daniel J., Greule, Anja, Cryle, Max J. and Tosin, Manuela (2021) Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis. Chemical Communications, 57 (18). pp. 2293-2296. doi:10.1039/D0CC07421G ISSN 1359-7345.

2019

Tosin, Manuela, Kilgour, Samantha Louise, Kilgour, David P. A., Prasongpholchai, Panward and O'Connor, Peter B. (2019) A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis. Chemistry – A European Journal, 25 (75). pp. 16515-16518. doi:10.1002/chem.201903662 ISSN 1521-3765.

Tosin, Manuela, Kilgour, Samantha Louise and Jenkins, Robert (2019) A photoactivatable small molecule probe for the in vivo capture of polyketide intermediates. Chemistry – A European Journal, 25 (72). pp. 16511-16514. doi:10.1002/chem.201903661 ISSN 1521-3765.

Little, Rory, Paiva, Fernanda C. R., Jenkins, Robert, Hong, Hui, Sun, Yuhui, Demydchuk, Yuliya, Samborskyy, Markiyan, Tosin, Manuela, Leeper, Finian J., Dias, Marcio Vinicius Bertacine and Leadlay, Peter F. (2019) Unexpected enzyme-catalysed [4+2] cycloaddition and rearrangement in polyether antibiotic biosynthesis. Nature Catalysis, 2 . pp. 1045-1054. doi:10.1038/s41929-019-0351-2 ISSN 2520-1158.

Alberti, Fabrizio, Leng, Daniel J., Wilkening, Ina, Song, Lijiang, Tosin, Manuela and Corre, Christophe (2019) Triggering the expression of a silent gene cluster from genetically intractable bacteria results in scleric acid discovery. Chemical Science, 10 (2). pp. 453-463. doi:10.1039/C8SC03814G ISSN 2041-6520.

2017

Ho, Y. T. Candace, Leng, Daniel J., Ghiringhelli, Francesca, Wilkening, Ina, Bushell, Dexter P., Köstner, Otto, Riva, Elena, Havemann, Judith, Passarella, Daniele and Tosin, Manuela (2017) Novel chemical probes for the investigation of nonribosomal peptide assembly. Chemical Communications, 53 (52). pp. 7088-7091. doi:10.1039/c7cc02427d ISSN 1359-7345.

Havemann, Judith, Yurkovich, Marie E., Jenkins, Robert, Harringer, Sophia, Tao, Weixin, Wen, Shishi, Sun, Yuhui, Leadlay, P. F. and Tosin, Manuela (2017) Chemical probing of thiotetronate bio-assembly. Chemical Communications, 53 (11). pp. 1912-1915. doi:10.1039/C6CC09933E ISSN 1359-7345.

Yurkovich, Marie E., Jenkins, Robert, Sun, Yuhui, Tosin, Manuela and Leadlay, P. F. (2017) The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase. Chemical Communications, 53 (13). pp. 2182-2185. doi:10.1039/C6CC09934C ISSN 1359-7345.

2016

Wilkening, Ina, Gazzola, Silvia, Riva, Elena, Parascandolo, James S., Song, Lijiang and Tosin, Manuela (2016) Second-generation probes for biosynthetic intermediate capture : towards a comprehensive profiling of polyketide assembly. Chemical Communications, 52 (68). 10392-10395 . doi:10.1039/C6CC04681A ISSN 1359-7345.

Parascandolo, James S., Havemann, Judith, Potter, Helen K., Huang, Fanglu, Riva, Elena, Connolly, Jack, Wilkening, Ina, Song, Lijiang, Leadlay, P. F. and Tosin, Manuela (2016) Insights into 6-methylsalicylic acid bio-assembly by using chemical probes. Angewandte Chemie International Edition, 55 (10). pp. 3463-3467. doi:10.1002/anie.201509038 ISSN 1433-7851.

Tao, W., Yurkovich, M. E., Wen, S., Lebe, K. E., Samborskyy, M., Liu, Y., Yang, A., Liu, Y., Ju, Y., Deng, Z., Tosin, Manuela, Sun, Y. and Leadlay, P. F. (2016) A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. Chemical Science, 7 (1). pp. 376-385. doi:10.1039/c5sc03059e ISSN 2041-6520.

2015

Kage, Hirokazu, Riva, Elena, Parascandolo, James S., Kreutzer, Martin F., Tosin, Manuela and Nett, Markus (2015) Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase. Organic & Biomolecular Chemistry, 13 (47). pp. 11414-11417. doi:10.1039/c5ob02009c ISSN 1477-0520.

2014

Riva, Elena, Wilkening, Ina, Gazzola, Silvia, Li, W. M. Ariel, Smith, Luke, Leadlay, P. F. and Tosin, Manuela (2014) Chemical probes for the functionalization of polyketide intermediates. Angewandte Chemie International Edition, 53 (44). pp. 11944-11949. doi:10.1002/anie.201407448 ISSN 1433-7851.

2012

Wills, Rebecca, Tosin, Manuela and O’Connor, Peter B. (2012) Structural characterization of polyketides using high mass accuracy tandem mass spectrometry. Analytical Chemistry, Volume 84 (Number 20). pp. 8863-8870. doi:10.1021/ac3022778 ISSN 0003-2700.

2011

Tosin, Manuela, Demydchuk, Yuliya, Parascandolo, James S., Blasco Per, Covadonga, Leeper, F. J. (Finian J.) and Leadlay, P. F. (2011) In vivo trapping of polyketide intermediates from an assembly line synthase using malonyl carba(dethia)-N-acetyl cysteamines. Chemical Communications, Vol.47 (No.12). pp. 3460-3462. doi:10.1039/c0cc05077f ISSN 1359-7345.

Tosin, Manuela, Smith, L. (Luke) and Leadlay, P. F. (2011) Insights into lasalocid a ring formation by chemical chain termination in vivo. Angewandte Chemie International Edition, Vol.50 (No.50). pp. 11930-11933. doi:10.1002/anie.201106323 ISSN 1433-7851.

Kwan, David H., Tosin, Manuela, Schläger, Nadin, Schulz, F. (Frank) and Leadlay, P. F. (2011) Insights into the stereospecificity of ketoreduction in a modular polyketide synthase. Organic & Biomolecular Chemistry, Vol.9 (No.7). pp. 2053-2056. doi:10.1039/c1ob00022e ISSN 1477-0520.

2010

Lazos, Orestis, Tosin, Manuela, Slusarczyk, Adrian L., Boakes, Steven, Cortés, Jesus, Sidebottom, Philip J. and Leadlay, P. F. (2010) Biosynthesis of the putative siderophore erythrochelin requires unprecedented crosstalk between separate nonribosomal peptide gene clusters. Chemistry & Biology, Vol.17 (No.2). pp. 160-173. doi:10.1016/j.chembiol.2010.01.011 ISSN 1074-5521.

Sun, Yuhui, Hahn, F., Demydchuk, Yuliya, Chettle, James, Tosin, Manuela, Osada, Hiroyuki and Leadlay, P. F. (2010) In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chemical Biology, Vol.6 (No.2). pp. 99-101. doi:10.1038/nchembio.285 ISSN 1552-4450.

Tran, Lucky, Broadhurst, R. William, Tosin, Manuela, Cavalli, Andrea and Weissman, Kira J. (2010) Insights into protein-protein and enzyme-substrate interactions in modular polyketide synthases. Chemistry & Biology, Vol.17 (No.7). pp. 705-716. doi:10.1016/j.chembiol.2010.05.017 ISSN 1074-5521.

Dias, M. V. B., Huang, Fanglu, Chirgadze, D. Y., Tosin, Manuela, Spiteller, Dieter, Dry, E. F. V., Leadlay, P. F., Spencer, Jonathan B. and Blundell, T. L. (Tom Leon) (2010) Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK. Journal of Biological Chemistry, Vol.285 (No.29). pp. 22495-22504. doi:10.1074/jbc.M110.107177 ISSN 0021-9258.

Tosin, Manuela, Betancor, Lorena, Stephens, E. (Elaine), Li, W. M. Ariel, Spencer, Jonathan B. and Leadlay, P. F. (2010) Synthetic chain terminators off-load intermediates from a type I polyketide synthase. Chembiochem, Vol.11 (No.4). pp. 539-546. doi:10.1002/cbic.200900772 ISSN 1439-4227.

2009

Tosin, Manuela, Spiteller, Dieter and Spencer, Jonathan B. (2009) Malonyl carba(dethia)- and Malonyl oxa(dethia)-coenzyme A as tools for trapping polyketide intermediates. Chembiochem, Vol.10 (No.10). pp. 1714-1723. doi:10.1002/cbic.200900093 ISSN 1439-4227.

André, Sabine, Velasco-Torrijos, Trinidad, Leyden, Rosaria, Gouin, Sebastien, Tosin, Manuela, Murphy, Paul V. and Gabius, H. J. (2009) Phenylenediamine-based bivalent glycocyclophanes : synthesis and analysis of the influence of scaffold rigidity and ligand spacing on lectin binding in cell systems with different glycomic profiles. Organic & Biomolecular Chemistry, Vol.7 (No.22). pp. 4715-4725. doi:10.1039/b913010a ISSN 1477-0520.

2008

Tran, Lucky, Tosin, Manuela, Spencer, Jonathan B., Leadlay, P. F. and Weissman, Kira J. (2008) Covalent linkage mediates communication between ACP and TE domains in modular polyketide synthases. Chembiochem, Vol.9 (No.6). pp. 905-915. doi:10.1002/cbic.200700738 ISSN 1439-4227.