(2019) Molecular evolutionary trends and feeding ecology diversification in the Hemiptera anchored by the milkweed bug genome. Genome Biology, 20 . 64. doi:10.1186/s13059-019-1660-0 ISSN 1474-7596.
Preview |
PDF
WRAP-molecular-evolutionary-trends-feeding-ecology-diversification-milkweed-Panfilio-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (5MB) | Preview |
Abstract
Background
The Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae.
Results
The 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted the evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid nutrition feeding.
Conclusions
With the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus’s strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history Q Science > QL Zoology S Agriculture > SB Plant culture |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | Plant diseases, RNA interference, Hemiptera , Molecular genetics |
| Journal or Publication Title: | Genome Biology |
| Publisher: | BioMed Central Ltd. |
| ISSN: | 1474-7596 |
| Official Date: | 2 April 2019 |
| Dates: | Date Event 2 April 2019 Available 21 February 2019 Accepted |
| Volume: | 20 |
| Article Number: | 64 |
| DOI: | 10.1186/s13059-019-1660-0 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 3 April 2019 |
| Date of first compliant Open Access: | 3 April 2019 |
| RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID 1003A_143936 [SNSF] Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung P00P3_170664 [SNSF] Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung RC-CoG #616346 European Research Council UNSPECIFIED |
| Related URLs: | |
| URI: | https://wrap.warwick.ac.uk/115066/ |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |