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Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces

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Ishibe, Toru, Congdon, Thomas R., Stubbs, Christopher D., Hasan, Muhammad, Sosso, Gabriele C. and Gibson, Matthew I. (2019) Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces. ACS Macro Letters, 8 (8). pp. 1063-1067. doi:10.1021/acsmacrolett.9b00386 ISSN 2161-1653.

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Official URL: http://dx.doi.org/10.1021/acsmacrolett.9b00386

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Abstract

Antifreeze (glyco) proteins (AF(G)Ps) are potent inhibitors of ice recrystallization and may have biotechnological applications. The most potent AF(G)Ps function at concentrations a thousand times lower than synthetic mimics such as poly(vinyl alcohol), PVA. Here, we demonstrate that PVA’s ice recrystallization activity can be rescued at concentrations where it does not normally function, by the addition of noninteracting polymeric depletants, due to PVA forming colloids in the concentrated saline environment present between ice crystals. These depletants shift the equilibrium toward ice binding and, hence, enable PVA to inhibit ice growth at lower concentrations. Using theory and experiments, we show this effect requires polymeric depletants, not small molecules, to enhance activity. These results increase our understanding of how to design new ice growth inhibitors, but also offer opportunities to enhance activity by exploiting depletion forces, without re-engineering ice-binding materials. It also shows that when screening for IRI activity that polymer contaminants in buffers may give rise to false positive results.

Item Type: Journal Article
Subjects: Q Science > QC Physics
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Antifreeze proteins, Ice crystals -- Growth
Journal or Publication Title: ACS Macro Letters
Publisher: American Chemical Society
ISSN: 2161-1653
Official Date: 20 August 2019
Dates:
DateEvent
20 August 2019Published
9 August 2019Available
7 August 2019Accepted
Volume: 8
Number: 8
Page Range: pp. 1063-1067
DOI: 10.1021/acsmacrolett.9b00386
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 21 August 2019
Date of first compliant Open Access: 30 August 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
638661European Research Councilhttp://dx.doi.org/10.13039/501100000781
PoC Grant (789182)European Research Councilhttp://dx.doi.org/10.13039/501100000781
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