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Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces
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Ishibe, Toru, Congdon, Thomas R., Stubbs, Christopher D., Hasan, Muhammad, Sosso, Gabriele C. and Gibson, Matthew I. (2019) Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces. ACS Macro Letters, 8 (8). pp. 1063-1067. doi:10.1021/acsmacrolett.9b00386
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WRAP-enhancement-macromolecular-ice-recrystallization-inhibition-activity-exploiting-depletion-forces-Gibson-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (2202Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acsmacrolett.9b00386
Abstract
Antifreeze (glyco) proteins (AF(G)Ps) are potent inhibitors of ice recrystallization and may have biotechnological applications. The most potent AF(G)Ps function at concentrations a thousand times lower than synthetic mimics such as poly(vinyl alcohol), PVA. Here, we demonstrate that PVA’s ice recrystallization activity can be rescued at concentrations where it does not normally function, by the addition of noninteracting polymeric depletants, due to PVA forming colloids in the concentrated saline environment present between ice crystals. These depletants shift the equilibrium toward ice binding and, hence, enable PVA to inhibit ice growth at lower concentrations. Using theory and experiments, we show this effect requires polymeric depletants, not small molecules, to enhance activity. These results increase our understanding of how to design new ice growth inhibitors, but also offer opportunities to enhance activity by exploiting depletion forces, without re-engineering ice-binding materials. It also shows that when screening for IRI activity that polymer contaminants in buffers may give rise to false positive results.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QC Physics Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Antifreeze proteins, Ice crystals -- Growth | |||||||||
| Journal or Publication Title: | ACS Macro Letters | |||||||||
| Publisher: | American Chemical Society | |||||||||
| ISSN: | 2161-1653 | |||||||||
| Official Date: | 20 August 2019 | |||||||||
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| Volume: | 8 | |||||||||
| Number: | 8 | |||||||||
| Page Range: | pp. 1063-1067 | |||||||||
| DOI: | 10.1021/acsmacrolett.9b00386 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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