Carter, Simon J., Ferecskó, Alex S., King, Lloyd, Ménochet, Karelle, Parton, Ted and Chappell, Michael J. (2019) A mechanistic modelling approach for the determination of the mechanisms of inhibition by cyclosporine on the uptake and metabolism of atorvastatin in rat hepatocytes using a high throughput uptake method. Xenobiotica . doi:10.1080/00498254.2019.1652781 ISSN 1366-5928.
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Abstract
Determine the inhibition mechanism through which cyclosporine inhibits the uptake and metabolism of atorvastatin in fresh rat hepatocytes using mechanistic models applied to data generated using a high throughput oil spin method.
Atorvastatin was incubated in fresh rat hepatocytes (0.05–150 nmol/ml) with or without 20 min pre-incubation with 10 nmol/ml cyclosporine and sampled over 0.25–60 min using a high throughput oil spin method. Micro-rate constant and macro-rate constant mechanistic models were ranked based on goodness of fit values.
The best fitting model to the data was a micro-rate constant mechanistic model including non-competitive inhibition of uptake and competitive inhibition of metabolism by cyclosporine (Model 2). The association rate constant for atorvastatin was 150-fold greater than the dissociation rate constant and 10-fold greater than the translocation into the cell. The association and dissociation rate constants for cyclosporine were 7-fold smaller and 10-fold greater, respectively, than atorvastatin. The simulated atorvastatin-transporter-cyclosporine complex derived using the micro-rate constant parameter estimates increased in line with the incubation concentration of atorvastatin.
The increased amount of data generated with the high throughput oil spin method, combined with a micro-rate constant mechanistic model helps to explain the inhibition of uptake by cyclosporine following pre-incubation.
Item Type: | Journal Article |
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Subjects: | Q Science > QP Physiology R Medicine > RM Therapeutics. Pharmacology |
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering |
SWORD Depositor: | Library Publications Router |
Library of Congress Subject Headings (LCSH): | Liver cells -- Computer simulation, Cyclosporine -- Synthesis -- Inhibitors -- Computer simulation, Statins (Cardiovascular agents) |
Journal or Publication Title: | Xenobiotica |
Publisher: | Informa UK Limited |
ISSN: | 1366-5928 |
Official Date: | 19 August 2019 |
Dates: | Date Event 19 August 2019 Published 7 August 2019 Accepted |
DOI: | 10.1080/00498254.2019.1652781 |
Status: | Peer Reviewed |
Publication Status: | Published |
Access rights to Published version: | Open Access (Creative Commons open licence) |
Date of first compliant deposit: | 27 September 2019 |
Date of first compliant Open Access: | 27 September 2019 |
RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID 1548253 [BBSRC] Biotechnology and Biological Sciences Research Council |
Related URLs: | |
URI: | https://wrap.warwick.ac.uk/125921/ |
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