Hill, Emily, Dale, Nicholas and Wall, Mark J. (2020) Detecting CO2-sensitive hemichannels in neurons in acute brain slices. STAR Protocols, 1 (3). 100139. doi:10.1016/j.xpro.2020.100139 ISSN 2666-1667.
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Abstract
This protocol provides two independent methods to functionally detect the neuronal expression of CO2-sensitive hemichannels. These hemichannels (consisting of connexins 26 or 30) are directly gated by CO2, independent of pH changes and until recently were thought to be only expressed by glia. This protocol outlines a method to change the concentration of CO2 without changing pH, using isohydric solutions and then utilizing this to detect opening and closing of functional hemichannels using whole-cell patch clamp recording and dye loading.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QP Physiology |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | Neurons, Astrocytes, Nervous system, Brain -- Physiology, Connexins |
| Journal or Publication Title: | STAR Protocols |
| Publisher: | Cell Press |
| ISSN: | 2666-1667 |
| Official Date: | 18 December 2020 |
| Dates: | Date Event 18 December 2020 Published 23 October 2020 Available 30 September 2020 Accepted |
| Volume: | 1 |
| Number: | 3 |
| Article Number: | 100139 |
| DOI: | 10.1016/j.xpro.2020.100139 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 12 October 2020 |
| Date of first compliant Open Access: | 14 October 2020 |
| RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID UNSPECIFIED [BBSRC] Biotechnology and Biological Sciences Research Council |
| Related URLs: | |
| URI: | https://wrap.warwick.ac.uk/142886/ |
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