Gurska, Daniela, Vargas Jentzsch, Iris M. and Panfilio, Kristen A. (2020) Unexpected mutual regulation underlies paralogue functional diversification and promotes epithelial tissue maturation in Tribolium. Communications Biology, 3 (1). 552. doi:10.1038/s42003-020-01250-3 ISSN 2399-3642.
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Abstract
Insect Hox3/zen genes represent an evolutionary hotspot for changes in function and copy number. Single orthologues are required either for early specification or late morphogenesis of the extraembryonic tissues, which protect the embryo. The tandemly duplicated zen paralogues of the beetle Tribolium castaneum present a unique opportunity to investigate both functions in a single species. We dissect the paralogues' expression dynamics (transcript and protein) and transcriptional targets (RNA-seq after RNAi) throughout embryogenesis. We identify an unexpected role of Tc-Zen2 in repression of Tc-zen1, generating a negative feedback loop that promotes developmental progression. Tc-Zen2 regulation is dynamic, including within co-expressed multigene loci. We also show that extraembryonic development is the major event within the transcriptional landscape of late embryogenesis and provide a global molecular characterization of the extraembryonic serosal tissue. Altogether, we propose that paralogue mutual regulation arose through multiple instances of zen subfunctionalization, leading to their complementary extant roles.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
| SWORD Depositor: | Library Publications Router |
| Library of Congress Subject Headings (LCSH): | Evolutionary genetics, Red flour beetle -- Genetics, Macroevolution |
| Journal or Publication Title: | Communications Biology |
| Publisher: | Nature Publishing Group |
| ISSN: | 2399-3642 |
| Official Date: | 5 October 2020 |
| Dates: | Date Event 5 October 2020 Published 21 August 2020 Accepted |
| Volume: | 3 |
| Number: | 1 |
| Article Number: | 552 |
| DOI: | 10.1038/s42003-020-01250-3 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Re-use Statement: | ** From PubMed via Jisc Publications Router ** History: received 19-09-2019; accepted 21-08-2020. |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 26 October 2020 |
| Date of first compliant Open Access: | 26 October 2020 |
| RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID |
| URI: | https://wrap.warwick.ac.uk/143249/ |
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