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Biological insights from the direct measurement of purine release

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Dale, Nicholas (2021) Biological insights from the direct measurement of purine release. Biochemical Pharmacology, 187 . 114416. doi:10.1016/j.bcp.2021.114416 ISSN 0006-2952.

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Official URL: http://dx.doi.org/10.1016/j.bcp.2021.114416

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Abstract

Although purinergic signalling has been a well-established and accepted mechanism of chemical communication for many years, it remains important to measure the extracellular concentration of ATP and adenosine in real time. In this review I summarize the reasons why such measurements are still needed, how they provide additional mechanistic insight and give an overview of the techniques currently available to make spatially localised measurements of ATP and adenosine in real time. To illustrate the impact of direct real-time measurements, I explore CO2 and nutrient sensing in the medulla oblongata and hypothalamus. In both of these examples, the sensing involves hemichannel mediated ATP release from glial cells. For CO2 the hemichannels involved, connexin26, are directly CO2-sensitive. This mechanism contributes to the chemosensory control of breathing. In the hypothamalus, specialised glial cells, tanycytes, directly contact the cerebrospinal fluid in the 3rd ventricle and sense nutrients via sweet and umami taste receptors. Nutrient sensing by tanycytes is likely to contribute to the control of body weight as their selective stimulation alters food intake. To illustrate the importance of direct adenosine measurements, I consider the complex and multiple mechanisms of activity-dependent adenosine release in different brain regions. This activity dependent release of adenosine is likely to mediate important feedback regulation and may also be involved in controlling the sleep-wake state. I finish by briefly considering the potential of whole blood purine measurements in clinical practice.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Adenosine triphosphate , Biosensors , Connexins , Purines , Purines -- Receptors
Journal or Publication Title: Biochemical Pharmacology
Publisher: Elsevier Inc.
ISSN: 0006-2952
Official Date: May 2021
Dates:
DateEvent
May 2021Published
12 January 2021Available
7 January 2021Accepted
Volume: 187
Article Number: 114416
DOI: 10.1016/j.bcp.2021.114416
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 18 February 2021
Date of first compliant Open Access: 12 January 2022
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDWellcome Trusthttp://dx.doi.org/10.13039/100010269
UNSPECIFIED[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
UNSPECIFIED[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
Is Part Of: 1

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