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End-functionalized poly(vinylpyrrolidone) for ligand display in lateral flow device test lines
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Baker, Alexander N., Congdon, Thomas R., Richards, Sarah-Jane, Georgiou, Panagiotis G., Walker, Marc, Dedola, Simone, Field, Robert A. and Gibson, Matthew I. (2022) End-functionalized poly(vinylpyrrolidone) for ligand display in lateral flow device test lines. ACS Polymers Au, 2 (2). pp. 69-79. doi:10.1021/acspolymersau.1c00032 ISSN 2694-2453.
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Official URL: https://doi.org/10.1021/acspolymersau.1c00032
Abstract
Lateral flow devices are rapid (and often low cost) point-of-care diagnostics─the classic example being the home pregnancy test. A test line (the stationary phase) is typically prepared by the physisorption of an antibody, which binds to analytes/antigens such as viruses, toxins, or hormones. However, there is no intrinsic requirement for the detection unit to be an antibody, and incorporating other ligand classes may bring new functionalities or detection capabilities. To enable other (nonprotein) ligands to be deployed in lateral flow devices, they must be physiosorbed to the stationary phase as a conjugate, which currently would be a high-molecular-weight carrier protein, which requires (challenging) chemoselective modifications and purification. Here, we demonstrate that poly(vinylpyrrolidone), PVP, is a candidate for a polymeric, protein-free test line, owing to its unique balance of water solubility (for printing) and adhesion to the nitrocellulose stationary phase. End-functionalized PVPs were prepared by RAFT polymerization, and the model capture ligands of biotin and galactosamine were installed on PVP and subsequently immobilized on nitrocellulose. This polymeric test line was validated in both flow-through and full lateral flow formats using streptavidin and soybean agglutinin and is the first demonstration of an “all-polymer” approach for installation of capture units. This work illustrates the potential of polymeric scaffolds as anchoring agents for small-molecule capture agents in the next generation of robust and modular lateral flow devices and that macromolecular engineering may provide real benefit.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QP Physiology | |||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Immunoassay, Immunoassay -- methods, Medical innovations, Medical technology, Biotechnology, Povidone, Biosensors | |||||||||||||||||||||
| Journal or Publication Title: | ACS Polymers Au | |||||||||||||||||||||
| Publisher: | American Chemical Society (ACS) | |||||||||||||||||||||
| ISSN: | 2694-2453 | |||||||||||||||||||||
| Official Date: | 13 April 2022 | |||||||||||||||||||||
| Dates: |
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| Volume: | 2 | |||||||||||||||||||||
| Number: | 2 | |||||||||||||||||||||
| Page Range: | pp. 69-79 | |||||||||||||||||||||
| DOI: | 10.1021/acspolymersau.1c00032 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 23 November 2021 | |||||||||||||||||||||
| Date of first compliant Open Access: | 25 November 2021 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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