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End-functionalized poly(vinylpyrrolidone) for ligand display in lateral flow device test lines

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Baker, Alexander N., Congdon, Thomas R., Richards, Sarah-Jane, Georgiou, Panagiotis G., Walker, Marc, Dedola, Simone, Field, Robert A. and Gibson, Matthew I. (2022) End-functionalized poly(vinylpyrrolidone) for ligand display in lateral flow device test lines. ACS Polymers Au, 2 (2). pp. 69-79. doi:10.1021/acspolymersau.1c00032 ISSN 2694-2453.

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Official URL: https://doi.org/10.1021/acspolymersau.1c00032

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Abstract

Lateral flow devices are rapid (and often low cost) point-of-care diagnostics─the classic example being the home pregnancy test. A test line (the stationary phase) is typically prepared by the physisorption of an antibody, which binds to analytes/antigens such as viruses, toxins, or hormones. However, there is no intrinsic requirement for the detection unit to be an antibody, and incorporating other ligand classes may bring new functionalities or detection capabilities. To enable other (nonprotein) ligands to be deployed in lateral flow devices, they must be physiosorbed to the stationary phase as a conjugate, which currently would be a high-molecular-weight carrier protein, which requires (challenging) chemoselective modifications and purification. Here, we demonstrate that poly(vinylpyrrolidone), PVP, is a candidate for a polymeric, protein-free test line, owing to its unique balance of water solubility (for printing) and adhesion to the nitrocellulose stationary phase. End-functionalized PVPs were prepared by RAFT polymerization, and the model capture ligands of biotin and galactosamine were installed on PVP and subsequently immobilized on nitrocellulose. This polymeric test line was validated in both flow-through and full lateral flow formats using streptavidin and soybean agglutinin and is the first demonstration of an “all-polymer” approach for installation of capture units. This work illustrates the potential of polymeric scaffolds as anchoring agents for small-molecule capture agents in the next generation of robust and modular lateral flow devices and that macromolecular engineering may provide real benefit.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Physics
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Immunoassay, Immunoassay -- methods, Medical innovations, Medical technology, Biotechnology, Povidone, Biosensors
Journal or Publication Title: ACS Polymers Au
Publisher: American Chemical Society (ACS)
ISSN: 2694-2453
Official Date: 13 April 2022
Dates:
DateEvent
13 April 2022Published
12 November 2021Available
26 October 2021Accepted
Volume: 2
Number: 2
Page Range: pp. 69-79
DOI: 10.1021/acspolymersau.1c00032
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 23 November 2021
Date of first compliant Open Access: 25 November 2021
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/M01116X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
814236H2020 Marie Skłodowska-Curie Actionshttp://dx.doi.org/10.13039/100010665
BB/M02878X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
EP/R511808/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/S506783/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
86605European Research Councilhttp://dx.doi.org/10.13039/501100000781
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