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Transcriptome analysis of Pseudomonas aeruginosa biofilm infection in an ex vivo pig model of the cystic fibrosis lung
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Harrington, Niamh, Littler, Jenny and Harrison, Freya (2022) Transcriptome analysis of Pseudomonas aeruginosa biofilm infection in an ex vivo pig model of the cystic fibrosis lung. Applied and Environmental Microbiology, 88 (3). e01789-21. doi:10.1128/AEM.01789-21 ISSN 0099-2240.
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Official URL: https://doi.org/10.1128/AEM.01789-21
Abstract
Pseudomonas aeruginosa is the predominant cause of chronic biofilm infections that form in the lungs of people with cystic fibrosis (CF). These infections are highly resistant to antibiotics and persist for years in the respiratory tract. One of the main research challenges is that current laboratory models do not accurately replicate key aspects of a P. aeruginosa biofilm infection, highlighted by previous RNA-sequencing studies. We compared the P. aeruginosa PA14 transcriptome in an ex vivo pig lung (EVPL) model of CF and a well-studied synthetic cystic fibrosis sputum medium (SCFM). P. aeruginosa was grown in the EVPL model for 1, 2 and 7 days, and in vitro in SCFM for 1 and 2 days. The RNA was extracted and sequenced at each time point. Our findings demonstrate that expression of antimicrobial resistance genes was cued by growth in the EVPL model, highlighting the importance of growth environment in determining accurate resistance profiles. The EVPL model created two distinct growth environments: tissue-associated biofilm and the SCFM surrounding tissue, each cued a transcriptome distinct from that seen in SCFM in vitro. The expression of quorum sensing associated genes in the EVPL tissue-associated biofilm at 48 h relative to in vitro SCFM was similar to CF sputum versus in vitro conditions. Hence, the EVPL model can replicate key aspects of in vivo biofilm infection that are missing from other current models. It provides a more accurate P. aeruginosa growth environment for determining antimicrobial resistance that quickly drives P. aeruginosa into a chronic-like infection phenotype.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
| Library of Congress Subject Headings (LCSH): | Pseudomonas aeruginosa -- Infections, Cystic fibrosis, Lungs -- Diseases, Biofilms, Messenger RNA, Quorum sensing (Microbiology), Drug resistance in microorganisms | |||||||||
| Journal or Publication Title: | Applied and Environmental Microbiology | |||||||||
| Publisher: | American Society for Microbiology | |||||||||
| ISSN: | 0099-2240 | |||||||||
| Official Date: | 8 February 2022 | |||||||||
| Dates: |
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| Volume: | 88 | |||||||||
| Number: | 3 | |||||||||
| Article Number: | e01789-21 | |||||||||
| DOI: | 10.1128/AEM.01789-21 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 3 December 2021 | |||||||||
| Date of first compliant Open Access: | 10 December 2021 | |||||||||
| RIOXX Funder/Project Grant: |
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