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Nucleolar targeting in an early-branching eukaryote suggests a general mechanism for ribosome protein sorting
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Jeilani, Milad, Billington, Karen, Sunter, Jack Daniel, Dean, Samuel and Wheeler, Richard John (2022) Nucleolar targeting in an early-branching eukaryote suggests a general mechanism for ribosome protein sorting. Journal of Cell Science, 135 (19). jcs259701. doi:10.1242/jcs.259701 ISSN 0021-9533.
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WRAP-nucleolar-targeting-early-branching-eukaryote-suggests-general-mechanism-ribosome-protein-sorting-Dean-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (6Mb) | Preview |
Official URL: https://doi.org/10.1242/jcs.259701
Abstract
The compartmentalised eukaryotic cell demands accurate targeting of proteins to the organelles in which they function, whether membrane-bound (like the nucleus) or non-membrane-bound (like the nucleolus). Nucleolar targeting relies on positively charged localisation signals and has received rejuvenated interest since the widespread recognition of liquid–liquid phase separation (LLPS) as a mechanism contributing to nucleolus formation. Here, we exploit a new genome-wide analysis of protein localisation in the early-branching eukaryote Trypanosoma brucei to analyse general nucleolar protein properties. T. brucei nucleolar proteins have similar properties to those in common model eukaryotes, specifically basic amino acids. Using protein truncations and addition of candidate targeting sequences to proteins, we show both homopolymer runs and distributed basic amino acids give nucleolar partition, further aided by a nuclear localisation signal (NLS). These findings are consistent with phase separation models of nucleolar formation and physical protein properties being a major contributing mechanism for eukaryotic nucleolar targeting, conserved from the last eukaryotic common ancestor. Importantly, cytoplasmic ribosome proteins, unlike mitochondrial ribosome proteins, have more basic residues – pointing to adaptation of physicochemical properties to assist segregation.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QL Zoology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Nucleolus , Trypanosoma -- Physiology, Mitochondria, Eukaryotic cells , Ribosomes | |||||||||
| Journal or Publication Title: | Journal of Cell Science | |||||||||
| Publisher: | The Company of Biologists Ltd. | |||||||||
| ISSN: | 0021-9533 | |||||||||
| Official Date: | 4 October 2022 | |||||||||
| Dates: |
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| Volume: | 135 | |||||||||
| Number: | 19 | |||||||||
| Article Number: | jcs259701 | |||||||||
| DOI: | 10.1242/jcs.259701 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 3 November 2022 | |||||||||
| Date of first compliant Open Access: | 4 November 2022 | |||||||||
| RIOXX Funder/Project Grant: |
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