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Investigation of mechanical properties of cancerous cells based on atomic force microscope
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Zhu, Jiajing (2022) Investigation of mechanical properties of cancerous cells based on atomic force microscope. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3850864
Abstract
Cancer is now responsible for the major leading cause of death worldwide. The late diagnosis of cancer greatly increases the difficulty of treatment and the mortality rate of patients. Therefore, effective early diagnosis and treatment of cancer have been urgent problems for the medical community to address. The detection of cancer and identification of drugs have become a promising and urgent work in a variety of disciplines, especially the biomechanics.
This thesis focused on the influences of measurement parameters on the measured central Young’s moduli of a single live A549 cell in vitro with atomic force microscope (AFM). Then, the comparison of the mechanical effects of sorafenib tosylate, osimertinib mesylate, herbal cinobufagin drug (HCD) and herbal Rg3 drug (HRD) on cancerous cells were investigated utilizing the force-volume (FV) mode and force spectroscopy (FS) mode of AFM. In addition, the morphological and mechanical properties of the interaction of cancerous cells with polyethylene terephthalate-based microlinear-structures (PET-MS) films and various conentrations of silver nanoparticles (AgNPs) were measured with AFM. Furthermore, the morphological and mechanical properties of five various live cells in vitro based on atomic force acoustic microscope (AFAM) are detected combining AFAM with inverted optical microscopy and scanning electron microscopy (SEM). The Sneddon model is applied to fit the force-distance curves and thus the mechanical properties of Young’s moduli can be calculated.
This thesis may have potential significance in providing references for the standardized detection of a single cancerous cell in vitro using AFM methodologies. The obtained results have provided insights into the classification and characterization of the effects of targeted drugs, herbal drugs, microlinear-structures and AgNPs on cancerous cells in vitro for cancer therapy. It is liable to furnish the multifunctional applicability of AFAM-based characterization in mechanobiology.
| Item Type: | Thesis (PhD) | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) T Technology > TA Engineering (General). Civil engineering (General) |
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| Library of Congress Subject Headings (LCSH): | Atomic force microscopy, Cancer -- Diagnosis, Cancer cells | ||||
| Official Date: | April 2022 | ||||
| Dates: |
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| Institution: | University of Warwick | ||||
| Theses Department: | School of Engineering | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | Tian, Yanling ; Liu, Xianping, 1957- | ||||
| Sponsors: | University of Warwick ; China Scholarship Council ; Horizon 2020 (Programme) | ||||
| Format of File: | |||||
| Extent: | xiii, ix, 179 leaves : illustrations (colour), charts | ||||
| Language: | eng |
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