Parmenter, Christopher D. J. and Stoilova-McPhie, Svetla. (2008) Binding of recombinant human coagulation factor VIII to lipid nanotubes. FEBS Letters, Vol.582 (No.12). pp. 1657-1660. ISSN 0014-5793

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Abstract

Cryo-electron microscopy has the power to visualise lipid membranes at the closest to in vivo conditions. The structure of the lipid bilayer can be well resolved and the interactions between lipid protein and protein protein molecules followed at the molecular level. We undertook an extended Cryoelectron microscopy study to follow the factor VIII binding to phosphatidylserine containing lipid nanotubes at different lipid composition. Obtaining well ordered tubes is required to define the factor VIII membrane bound structure. The observed alterations in the arrangement of the protein molecules are indicative for the flexibility of the membrane-bound factor VIII. Understanding the significance of these conformational changes is essential to comprehend the function of factor VIII in coagulation and as a drug for Hemophilia A. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Blood coagulation factors, Nanotubes, Membrane lipids, Membrane proteins, Protein binding, Cryomicroscopy, Electron microscopy
Journal or Publication Title:	FEBS Letters
Publisher:	Elsevier BV
ISSN:	0014-5793
Date:	28 May 2008
Volume:	Vol.582
Number:	No.12
Number of Pages:	4
Page Range:	pp. 1657-1660
Identification Number:	10.1016/j.febslet.2008.04.018
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	British Heart Foundation, Wellcome Trust (London, England)
Grant number:	PG/04/070 (BHF), 055663/Z/98/Z (WT)
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URI:	http://wrap.warwick.ac.uk/id/eprint/29639

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