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Single-headed mode of kinesin-5
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Kaseda, Kuniyoshi, Crevel, Isabelle, Hirose, Keiko and Cross, R. A. (2008) Single-headed mode of kinesin-5. EMBO reports, Vol.9 (No.8). pp. 761-765. doi:10.1038/embor.2008.96 ISSN 1469-221X.
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Official URL: http://dx.doi.org/10.1038/embor.2008.96
Abstract
In most organisms, kinesin-5 motors are essential for mitosis and meiosis, where they crosslink and slide apart the antiparallel microtubule half-spindles. Recently, it was shown using single-molecule optical trapping that a truncated, double-headed human kinesin-5 dimer can step processively along microtubules. However, processivity is limited (~8 steps) with little coordination between the heads, raising the possibility that kinesin-5 motors might also be able to move by a nonprocessive mechanism. To investigate this, we engineered single-headed kinesin-5 dimers. We show that a set of these single-headed Eg5 dimers drive microtubule sliding at about 90% of wild-type velocity, indicating that Eg5 can slide microtubules by a mechanism in which one head of each Eg5 head-pair is effectively redundant. On the basis of this, we propose a muscle-like model for Eg5-driven microtubule sliding in spindles in which most force-generating events are single-headed interactions and alternate-heads processivity is rare.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Kinesin, Microtubules, Cells -- Motility | ||||
| Journal or Publication Title: | EMBO reports | ||||
| Publisher: | Nature Publishing Group | ||||
| ISSN: | 1469-221X | ||||
| Official Date: | August 2008 | ||||
| Dates: |
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| Volume: | Vol.9 | ||||
| Number: | No.8 | ||||
| Page Range: | pp. 761-765 | ||||
| DOI: | 10.1038/embor.2008.96 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Japan Society for the Promotion of Science. Research Fellowship for Young Scientists , Marie Curie Cancer Care |
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