Single-headed mode of kinesin-5
Kaseda, Kuniyoshi, Crevel, Isabelle, Hirose, Keiko and Cross, R. A.. (2008) Single-headed mode of kinesin-5. EMBO reports, Vol.9 (No.8). pp. 761-765. ISSN 1469-221XFull text not available from this repository.
Official URL: http://dx.doi.org/10.1038/embor.2008.96
In most organisms, kinesin-5 motors are essential for mitosis and meiosis, where they crosslink and slide apart the antiparallel microtubule half-spindles. Recently, it was shown using single-molecule optical trapping that a truncated, double-headed human kinesin-5 dimer can step processively along microtubules. However, processivity is limited (~8 steps) with little coordination between the heads, raising the possibility that kinesin-5 motors might also be able to move by a nonprocessive mechanism. To investigate this, we engineered single-headed kinesin-5 dimers. We show that a set of these single-headed Eg5 dimers drive microtubule sliding at about 90% of wild-type velocity, indicating that Eg5 can slide microtubules by a mechanism in which one head of each Eg5 head-pair is effectively redundant. On the basis of this, we propose a muscle-like model for Eg5-driven microtubule sliding in spindles in which most force-generating events are single-headed interactions and alternate-heads processivity is rare.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH426 Genetics
Q Science > QP Physiology
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Kinesin, Microtubules, Cells -- Motility|
|Journal or Publication Title:||EMBO reports|
|Publisher:||Nature Publishing Group|
|Page Range:||pp. 761-765|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Japan Society for the Promotion of Science. Research Fellowship for Young Scientists , Marie Curie Cancer Care|
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