Kaseda, Kuniyoshi, Crevel, Isabelle, Hirose, Keiko and Cross, R. A.. (2008) Single-headed mode of kinesin-5. EMBO reports, Vol.9 (No.8). pp. 761-765. ISSN 1469-221X

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Abstract

In most organisms, kinesin-5 motors are essential for mitosis and meiosis, where they crosslink and slide apart the antiparallel microtubule half-spindles. Recently, it was shown using single-molecule optical trapping that a truncated, double-headed human kinesin-5 dimer can step processively along microtubules. However, processivity is limited (~8 steps) with little coordination between the heads, raising the possibility that kinesin-5 motors might also be able to move by a nonprocessive mechanism. To investigate this, we engineered single-headed kinesin-5 dimers. We show that a set of these single-headed Eg5 dimers drive microtubule sliding at about 90% of wild-type velocity, indicating that Eg5 can slide microtubules by a mechanism in which one head of each Eg5 head-pair is effectively redundant. On the basis of this, we propose a muscle-like model for Eg5-driven microtubule sliding in spindles in which most force-generating events are single-headed interactions and alternate-heads processivity is rare.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Kinesin, Microtubules, Cells -- Motility
Journal or Publication Title:	EMBO reports
Publisher:	Nature Publishing Group
ISSN:	1469-221X
Date:	August 2008
Volume:	Vol.9
Number:	No.8
Page Range:	pp. 761-765
Identification Number:	10.1038/embor.2008.96
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Japan Society for the Promotion of Science. Research Fellowship for Young Scientists , Marie Curie Cancer Care
URI:	http://wrap.warwick.ac.uk/id/eprint/36905

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