Adenoviruses: Molecular Biology
Leppard, Keith (2008) Adenoviruses: Molecular Biology. In: Mahy, Brian W. J. and Van Regenmortel, Marc H. V., (eds.) Encyclopedia of Virology. Amsterdam ; London: Elsevier Ltd., pp. 17-23. ISBN 9780123739360Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/B978-012374410-4.00356-3
The molecular biology of adenoviruses has been studied mainly using two human serotypes, 2 and 5. The virions are nonenveloped, icosahedral particles that contain a linear, double-stranded DNA genome of approximately 36 000 bp. Protein fibers project from the vertices of the particle, and these mediate primary attachment to the target cell. Cell entry is via receptor-mediated endocytosis. Following escape from the endosome, the partially uncoated capsid is transported to the nuclear surface where the DNA enters the nucleus. Transcription of mRNAs occurs in temporal phases, using host RNA polymerase II. Proteins produced early after infection modify the host environment, including mitigating host antiviral responses, and provide essential DNA replication functions, including DNA polymerase. Once replication has begun, high levels of late proteins are made, from which progeny particles are assembled. Detailed mechanisms of particle release have not been determined. In established cell lines, this cycle takes approximately 24 h, and considerably longer in primary cells. The detailed understanding of adenovirus replication is being used to develop this virus as a gene delivery vector.
Copyright © 2008 Elsevier Ltd. All rights reserved.
|Item Type:||Book Item|
|Subjects:||Q Science > Q Science (General)|
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Journal or Publication Title:||Adenoviridae: molecular biology|
|Place of Publication:||Amsterdam ; London|
|Book Title:||Encyclopedia of Virology|
|Editor:||Mahy, Brian W. J. and Van Regenmortel, Marc H. V.|
|Number of Pages:||7|
|Page Range:||pp. 17-23|
|Status:||Not Peer Reviewed|
|Access rights to Published version:||Restricted or Subscription Access|
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