Damianoglou, Angeliki, Rodger, Alison, Pridmore, Catherine, Dafforn, Tim, Mosely, Jackie A., Sanderson, John M. and Hicks, Matthew R.. (2010) The synergistic action of melittin and phospholipase A2 with lipid membranes : development of linear dichroism for membrane-insertion kinetics. Protein and Peptide Letters, Vol.17 (No.11). pp. 1351-1362. ISSN 0929-8665

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Abstract

Here we present data on the kinetics of insertion of melittin, a peptide from bee venom, into lipid membranes of different composition. Another component of bee venom is the enzyme phospholipase A2 (PLA(2)). We have examined the interaction of melittin and PLA(2) with liposomes both separately and combined and demonstrate that they work synergistically to disrupt the membranes. A dramatic difference in the action of melittin and PLA(2) is observed when the composition of the membrane is altered. Temperature also has a large effect on the kinetics of insertion and membrane disruption. We use a combination of techniques to measure liposome size (dynamic light scattering), peptide secondary structure (circular dichroism spectroscopy), peptide orientation relative to the membrane (linear dichroism spectroscopy) and enzymatic digestion of the lipids (mass spectrometry).

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Linear dichroism, Phospholipase A2, Membrane proteins, Peptides, Liposomes
Journal or Publication Title:	Protein and Peptide Letters
Publisher:	Bentham Science Publishers Ltd.
ISSN:	0929-8665
Date:	November 2010
Volume:	Vol.17
Number:	No.11
Number of Pages:	12
Page Range:	pp. 1351-1362
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/4731

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