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The synergistic action of melittin and phospholipase A2 with lipid membranes : development of linear dichroism for membrane-insertion kinetics
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Damianoglou, Angeliki, Rodger, Alison, Pridmore, Catherine, Dafforn, Tim, Mosely, Jackie A., Sanderson, John M. and Hicks, Matthew R.. (2010) The synergistic action of melittin and phospholipase A2 with lipid membranes : development of linear dichroism for membrane-insertion kinetics. Protein and Peptide Letters, Vol.17 (No.11). pp. 1351-1362. ISSN 0929-8665
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Official URL: http://www.bentham.org/ppl
Abstract
Here we present data on the kinetics of insertion of melittin, a peptide from bee venom, into lipid membranes of different composition. Another component of bee venom is the enzyme phospholipase A2 (PLA(2)). We have examined the interaction of melittin and PLA(2) with liposomes both separately and combined and demonstrate that they work synergistically to disrupt the membranes. A dramatic difference in the action of melittin and PLA(2) is observed when the composition of the membrane is altered. Temperature also has a large effect on the kinetics of insertion and membrane disruption. We use a combination of techniques to measure liposome size (dynamic light scattering), peptide secondary structure (circular dichroism spectroscopy), peptide orientation relative to the membrane (linear dichroism spectroscopy) and enzymatic digestion of the lipids (mass spectrometry).
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
| Divisions: | Faculty of Science > Chemistry |
| Library of Congress Subject Headings (LCSH): | Linear dichroism, Phospholipase A2, Membrane proteins, Peptides, Liposomes |
| Journal or Publication Title: | Protein and Peptide Letters |
| Publisher: | Bentham Science Publishers Ltd. |
| ISSN: | 0929-8665 |
| Official Date: | November 2010 |
| Volume: | Vol.17 |
| Number: | No.11 |
| Number of Pages: | 12 |
| Page Range: | pp. 1351-1362 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/4731 |
Data sourced from Thomson Reuters' Web of Knowledge
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