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A rapid, reliable method for uNK cell density estimation
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Drury, Josephine A., Tang, Ai-Wei, Turner, Mark A. and Quenby, Siobhan (2013) A rapid, reliable method for uNK cell density estimation. Journal of Reproductive Immunology, Volume 97 (Number 2). pp. 183-185. doi:10.1016/j.jri.2012.12.002 ISSN 0165-0378.
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Official URL: http://dx.doi.org/10.1016/j.jri.2012.12.002
Abstract
Interest in uterine NK cell density as a diagnostic or screening tool in reproductive disorders is growing. However, current methods of analysis are time consuming. In this study, 997 images from 100 women were analysed both by the currently used method combining manual and computer aided counting, and by using colour splitting combined with area measurement as an estimate of positively stained cells. Good correlation was found between both methods (r2 = 0.92) centred around the line of equality, with no systematic differences observed in Bland Altman plots. The area measurement was significantly faster and thus is a useful screening method.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology > QR180 Immunology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Killer cells, Uterus -- Immunology, Endometrium, Immunohistochemistry, Image analysis | ||||
| Journal or Publication Title: | Journal of Reproductive Immunology | ||||
| Publisher: | Elsevier Ireland Ltd | ||||
| ISSN: | 0165-0378 | ||||
| Official Date: | April 2013 | ||||
| Dates: |
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| Volume: | Volume 97 | ||||
| Number: | Number 2 | ||||
| Page Range: | pp. 183-185 | ||||
| DOI: | 10.1016/j.jri.2012.12.002 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Description: | Short communication |
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