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Isolation of environmental lignin-degrading bacteria and identification of extracellular enzymes
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Taylor, C. R. (Charles R.) (2013) Isolation of environmental lignin-degrading bacteria and identification of extracellular enzymes. PhD thesis, University of Warwick.
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WRAP_THESIS_Taylor_2013.pdf - Submitted Version Download (26Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b2686753~S1
Abstract
A novel screening method for detecting lignin-degradation activity on agar
plates was developed using nitrated lignin. Using this method, ten lignindegrading
bacteria have been isolated from environmental sources, including
seven mesophilic soil bacteria and three thermotolerant strains from composted
wheat straw. All of the isolates have demonstrated activity towards lignin
degradation in the assays, the most active strain being a thermotolerant
Sphingobacterium strain from the Bacteroidetes family.
The ability of each strain to degrade a variety of aromatic carbon sources and
size-fractionated Kraft lignin has been examined by laboratory-scale growth
experiments and gel filtration chromatography respectively, and the
bioconversion of different lignin-containing feedstocks by three of the most
active strains has been examined in a series of laboratory-scale fermentation
experiments.
Purification of extracellular lignin-degrading enzymes from the culture
supernatant of Sphingobacterium sp. has highlighted several different enzyme
activities and possible lignin-degrading enzymes.
| Item Type: | Thesis or Dissertation (PhD) | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
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| Library of Congress Subject Headings (LCSH): | Lignin -- Biodegradation, Isolating mechanisms, Extracellular enzymes | ||||
| Official Date: | April 2013 | ||||
| Dates: |
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| Institution: | University of Warwick | ||||
| Theses Department: | Department of Chemistry | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | Bugg, Tim | ||||
| Sponsors: | Fondation Tuck | ||||
| Extent: | 221 leaves : illustrations. | ||||
| Language: | eng |
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