Katsuki, Miho, Drummond, Douglas R. and Cross, R. A. (2014) Ectopic A-lattice seams destabilize microtubules. Nature Communications, Volume 5 . Article number 3094. doi:10.1038/ncomms4094 ISSN 2041-1723.

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Abstract

Natural microtubules typically include one A-lattice seam within an otherwise helically symmetric B-lattice tube. It is currently unclear how A-lattice seams influence microtubule dynamic instability. Here we find that including extra A-lattice seams in GMPCPP microtubules, structural analogues of the GTP caps of dynamic microtubules, destabilizes them, enhancing their median shrinkage rate by >20-fold. Dynamic microtubules nucleated by seeds containing extra A-lattice seams have growth rates similar to microtubules nucleated by B-lattice seeds, yet have increased catastrophe frequencies at both ends. Furthermore, binding B-lattice GDP microtubules to a rigor kinesin surface stabilizes them against shrinkage, whereas microtubules with extra A-lattice seams are stabilized only slightly. Our data suggest that introducing extra A-lattice seams into dynamic microtubules destabilizes them by destabilizing their GTP caps. On this basis, we propose that the single A-lattice seam of natural B-lattice MTs may act as a trigger point, and potentially a regulation point, for catastrophe.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history
Divisions:	Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Microtubules
Journal or Publication Title:	Nature Communications
Publisher:	Nature Publishing Group
ISSN:	2041-1723
Official Date:	27 February 2014
Dates:	Date Event 27 February 2014 Published
Volume:	Volume 5
Number of Pages:	13
Article Number:	Article number 3094
DOI:	10.1038/ncomms4094
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	26 December 2015
Date of first compliant Open Access:	26 December 2015
Funder:	Association for International Cancer Research (AICR), Marie Curie Cancer Care
Grant number:	09-0221 (AICR)

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