
The Library
Ectopic A-lattice seams destabilize microtubules
Tools
Katsuki, Miho, Drummond, Douglas R. and Cross, R. A. (2014) Ectopic A-lattice seams destabilize microtubules. Nature Communications, Volume 5 . Article number 3094. doi:10.1038/ncomms4094 ISSN 2041-1723.
|
Text
WRAP_Drummond_ncomms4094.pdf - Published Version Available under License Creative Commons Attribution. Download (2306Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/ncomms4094
Abstract
Natural microtubules typically include one A-lattice seam within an otherwise helically symmetric B-lattice tube. It is currently unclear how A-lattice seams influence microtubule dynamic instability. Here we find that including extra A-lattice seams in GMPCPP microtubules, structural analogues of the GTP caps of dynamic microtubules, destabilizes them, enhancing their median shrinkage rate by >20-fold. Dynamic microtubules nucleated by seeds containing extra A-lattice seams have growth rates similar to microtubules nucleated by B-lattice seeds, yet have increased catastrophe frequencies at both ends. Furthermore, binding B-lattice GDP microtubules to a rigor kinesin surface stabilizes them against shrinkage, whereas microtubules with extra A-lattice seams are stabilized only slightly. Our data suggest that introducing extra A-lattice seams into dynamic microtubules destabilizes them by destabilizing their GTP caps. On this basis, we propose that the single A-lattice seam of natural B-lattice MTs may act as a trigger point, and potentially a regulation point, for catastrophe.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Library of Congress Subject Headings (LCSH): | Microtubules | ||||
Journal or Publication Title: | Nature Communications | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 2041-1723 | ||||
Official Date: | 27 February 2014 | ||||
Dates: |
|
||||
Volume: | Volume 5 | ||||
Number of Pages: | 13 | ||||
Article Number: | Article number 3094 | ||||
DOI: | 10.1038/ncomms4094 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 26 December 2015 | ||||
Date of first compliant Open Access: | 26 December 2015 | ||||
Funder: | Association for International Cancer Research (AICR), Marie Curie Cancer Care | ||||
Grant number: | 09-0221 (AICR) |
Request changes or add full text files to a record
Repository staff actions (login required)
![]() |
View Item |
Downloads
Downloads per month over past year