Kaur, Satdip, Fielding, Andrew B., Gassner, Gisela, Carter, Nicholas J. and Royle, Stephen J. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis. eLife, Volume 3 . Article number e00829. doi:10.7554/eLife.00829 ISSN 2050-084X.
Preview |
PDF
WRAP_Kaur_e00829.full.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution . Download (4MB) | Preview |
Abstract
Clathrin-mediated endocytosis (CME) is the major internalisation route for many different receptor types in mammalian cells. CME is shut down during early mitosis, but the mechanism of this inhibition is unclear. In this study, we show that the mitotic shutdown is due to an unmet requirement for actin in CME. In mitotic cells, membrane tension is increased and this invokes a requirement for the actin cytoskeleton to assist the CME machinery to overcome the increased load. However, the actin cytoskeleton is engaged in the formation of a rigid cortex in mitotic cells and is therefore unavailable for deployment. We demonstrate that CME can be 'restarted' in mitotic cells despite high membrane tension, by allowing actin to engage in endocytosis. Mitotic phosphorylation of endocytic proteins is maintained in mitotic cells with restored CME, indicating that direct phosphorylation of the CME machinery does not account for shutdown.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QP Physiology |
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Endocytosis, Actin, Mitosis |
| Journal or Publication Title: | eLife |
| Publisher: | eLife Sciences Publications Ltd. |
| ISSN: | 2050-084X |
| Official Date: | 18 February 2014 |
| Dates: | Date Event 18 February 2014 Published 8 January 2014 Accepted |
| Volume: | Volume 3 |
| Article Number: | Article number e00829 |
| DOI: | 10.7554/eLife.00829 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 28 July 2016 |
| Date of first compliant Open Access: | 28 July 2016 |
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Cancer Research UK (CRUK) |
| Grant number: | BB/H015582/1 (BBSRC), C25425/A15182 (CRUK) |
| URI: | https://wrap.warwick.ac.uk/59643/ |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |