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Cannabinoid receptors contribute to astroglial Ca2+-signalling and control of synaptic plasticity in the neocortex
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Rasooli-Nejad, Seyed, Palygin, Oleg, Lalo, Ulyana and Pankratov, Yuriy (2014) Cannabinoid receptors contribute to astroglial Ca2+-signalling and control of synaptic plasticity in the neocortex. Philosophical Transactions of the Royal Society B: Biological Sciences, Volume 369 (Number 1654). Article number 20140077. doi:10.1098/rstb.2014.0077 ISSN 0962-8436.
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Official URL: http://dx.doi.org/10.1098/rstb.2014.0077
Abstract
Communication between neuronal and glial cells is thought to be very important for many brain functions. Acting via release of gliotransmitters, astrocytes can modulate synaptic strength. The mechanisms underlying ATP release from astrocytes remain uncertain with exocytosis being the most intriguing and debated pathway. We have demonstrated that ATP and D-serine can be released from cortical astrocytes in situ by a SNARE-complex-dependent mechanism. Exocytosis of ATP from astrocytes can activate post-synaptic P2X receptors in the adjacent neurons, causing a downregulation of synaptic and extrasynaptic GABA receptors in cortical pyramidal neurons. We showed that release of gliotransmitters is important for the NMDA receptor-dependent synaptic plasticity in the neocortex. Firstly, induction of long-term potentiation (LTP) by five episodes of theta-burst stimulation (TBS) was impaired in the neocortex of dominant-negative (dn)-SNARE mice. The LTP was rescued in the dn-SNARE mice by application of exogenous non-hydrolysable ATP analogues. Secondly, we observed that weak sub-threshold stimulation (two TBS episodes) became able to induce LTP when astrocytes were additionally activated via CB-1 receptors. This facilitation was dependent on activity of ATP receptors and was abolished in the dn-SNARE mice. Our results strongly support the physiological relevance of glial exocytosis for glia–neuron communications and brain function.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Cannabinoids -- Receptors, Neuroplasticity, Synapses, Neocortex | ||||
Journal or Publication Title: | Philosophical Transactions of the Royal Society B: Biological Sciences | ||||
Publisher: | The Royal Society Publishing | ||||
ISSN: | 0962-8436 | ||||
Official Date: | 15 September 2014 | ||||
Dates: |
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Volume: | Volume 369 | ||||
Number: | Number 1654 | ||||
Article Number: | Article number 20140077 | ||||
DOI: | 10.1098/rstb.2014.0077 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | (BB/K009192/1) |
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