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Super-resolution kinetochore tracking reveals the mechanisms of human sister kinetochore directional switching
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Burroughs, Nigel John, Harry, Edward and McAinsh, Andrew D. (2015) Super-resolution kinetochore tracking reveals the mechanisms of human sister kinetochore directional switching. eLife, 4 . e09500. doi:10.7554/eLife.09500
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Official URL: http://dx.doi.org/10.7554/eLife.09500
Abstract
The congression of chromosomes to the spindle equator involves the directed motility of bi-orientated sister kinetochores. Sister kinetochores bind bundles of dynamic microtubules and are physically connected through centromeric chromatin. A crucial question is to understand how sister kinetochores are coordinated to generate motility and directional switches. Here, we combine super-resolution tracking of kinetochores with automated switching-point detection to analyse sister switching dynamics over thousands of events. We discover that switching is initiated by both the leading (microtubules depolymerising) or trailing (microtubules polymerising) kinetochore. Surprisingly, trail-driven switching generates an overstretch of the chromatin that relaxes over the following half-period. This rules out the involvement of a tension sensor, the central premise of the long-standing tension-model. Instead, our data support a model in which clocks set the intrinsic-switching time of the two kinetochore-attached microtubule fibres, with the centromeric spring tension operating as a feedback to slow or accelerate the clocks.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QH Natural history | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Spindle (Cell division), Microtubules, Chromatin, Centromere, Chromosomes | ||||||||
| Journal or Publication Title: | eLife | ||||||||
| Publisher: | eLife Sciences Publications Ltd. | ||||||||
| ISSN: | 2050-084X | ||||||||
| Official Date: | 13 October 2015 | ||||||||
| Dates: |
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| Volume: | 4 | ||||||||
| Article Number: | e09500 | ||||||||
| DOI: | 10.7554/eLife.09500 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England) | ||||||||
| Grant number: | BB/I021353/1 (BBSRC) 106151/Z/14/Z (Wellcome Trust) |
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