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Insights into 6-methylsalicylic acid bio-assembly by using chemical probes
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Parascandolo, James S., Havemann, Judith, Potter, Helen K., Huang, Fanglu, Riva, Elena, Connolly, Jack, Wilkening, Ina, Song, Lijiang, Leadlay, P. F. and Tosin, Manuela (2016) Insights into 6-methylsalicylic acid bio-assembly by using chemical probes. Angewandte Chemie International Edition, 55 (10). pp. 3463-3467. doi:10.1002/anie.201509038 ISSN 1433-7851.
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Official URL: http://dx.doi.org/10.1002/anie.201509038
Abstract
Chemical probes capable of reacting with KS (ketosynthase)-bound biosynthetic intermediates were utilized for the investigation of the model type I iterative polyketide synthase 6-methylsalicylic acid synthase (6-MSAS) in vivo and in vitro. From the fermentation of fungal and bacterial 6-MSAS hosts in the presence of chain termination probes, a full range of biosynthetic intermediates was isolated and characterized for the first time. Meanwhile, in vitro studies of recombinant 6-MSA synthases with both nonhydrolyzable and hydrolyzable substrate mimics have provided additional insights into substrate recognition, providing the basis for further exploration of the enzyme catalytic activities.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||
Library of Congress Subject Headings (LCSH): | Polyketides, Chemical inhibitors, Biosynthesis, Enzymatic analysis | ||||||||||||
Journal or Publication Title: | Angewandte Chemie International Edition | ||||||||||||
Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | ||||||||||||
ISSN: | 1433-7851 | ||||||||||||
Official Date: | 1 March 2016 | ||||||||||||
Dates: |
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Volume: | 55 | ||||||||||||
Number: | 10 | ||||||||||||
Page Range: | pp. 3463-3467 | ||||||||||||
DOI: | 10.1002/anie.201509038 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
Date of first compliant deposit: | 16 January 2019 | ||||||||||||
Date of first compliant Open Access: | 16 January 2019 | ||||||||||||
Funder: | University of Warwick. Institute of Advanced Study (IAS) | ||||||||||||
RIOXX Funder/Project Grant: |
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