Perry, David, Page, Ashley M., Chen, Baoping, Frenguelli, Bruno G. and Unwin, Patrick R. (2017) Differential-concentration scanning ion conductance microscopy. Analytical Chemistry, 89 (22). pp. 12458-12465. doi:10.1021/acs.analchem.7b03543 ISSN 0003-2700.
Preview |
PDF
WRAP-differential-concentration-scanning-ion-conductance-microscopy-Perry-2017.pdf - Accepted Version - Requires a PDF viewer. Download (1MB) | Preview |
Abstract
Scanning ion conductance microscopy (SICM) is a nanopipette-based scanning probe microscopy technique that utilizes the ionic current flowing between an electrode inserted inside a nanopipette probe containing electrolyte solution and a second electrode placed in a bulk electrolyte bath, to provide information on a substrate of interest. For most applications to date, the composition and concentration of the electrolyte inside and outside the nanopipette is identical, but it is shown herein that it can be very beneficial to lift this restriction. In particular, an ionic concentration gradient at the end of the nanopipette, generates an ionic current with a greatly reduced electric field strength, with particular benefits for live cell imaging. This differential concentration mode of SICM (ΔC-SICM) also enhances surface charge measurements and provides a new way to carry out reaction mapping measurements at surfaces using the tip for simultaneous delivery and sensing of the reaction rate. Comprehensive finite element method (FEM) modeling has been undertaken to enhance understanding of SICM as an electrochemical cell and to enable the interpretation and optimization of experiments. It is shown that electroosmotic flow (EOF) has much more influence on the nanopipette response in the ΔC-SICM configuration compared to standard SICM modes. The general model presented advances previous treatments, and it provides a framework for quantitative SICM studies.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC) |
| Library of Congress Subject Headings (LCSH): | Electrochemical apparatus, Electrochemical analysis |
| Journal or Publication Title: | Analytical Chemistry |
| Publisher: | American Chemical Society |
| ISSN: | 0003-2700 |
| Official Date: | 10 October 2017 |
| Dates: | Date Event 10 October 2017 Available 10 October 2017 Accepted |
| Volume: | 89 |
| Number: | 22 |
| Page Range: | pp. 12458-12465 |
| DOI: | 10.1021/acs.analchem.7b03543 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Date of first compliant deposit: | 22 November 2017 |
| Date of first compliant Open Access: | 10 October 2018 |
| RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID EP/F500378/1 Engineering and Physical Sciences Research Council UNSPECIFIED Warwick-China Scholarship Council UNSPECIFIED |
| URI: | https://wrap.warwick.ac.uk/95221/ |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |