The Library
An improved method for the rapid isolation of RNA from Arabidopsis and seeds of other species high in polyphenols and polysaccharides
Tools
Tools
Tools
Footitt, Steven, Awan, Sajjad Zahoor and Finch-Savage, William E. (2018) An improved method for the rapid isolation of RNA from Arabidopsis and seeds of other species high in polyphenols and polysaccharides. Seed Science Research, 28 (4). pp. 360-364. doi:10.1017/S0960258518000296 ISSN 0960-2585.
|
PDF
WRAP-improved-rapid-isolation-seeds-polysaccharides-Footitt-2018.pdf - Accepted Version - Requires a PDF viewer. Download (1008Kb) | Preview |
Official URL: https://doi.org/10.1017/S0960258518000296
Abstract
Seeds are notoriously high in polyphenols and polysaccharides, which reduce RNA quality and yield, and interfere with downstream applications. We present simple modifications to a rapid RNA extraction protocol for use with seeds. The inclusion of polyethylene glycol in place of polyvinylpyrrolidone reduced polyphenol and polysaccharide contamination. In addition, replacing NaCl with KCl improved the RNA yield from Arabidopsis seeds still bound by mucilage. On extraction of Arabidopsis seed recovered from field soils clean RNA pellets with no accompanying gelatinous matrix (polysaccharide) were seen, with A260/230 ratios greater than 1.8 confirming the lack of polysaccharide carry-over. When Brassica oleracea and Sinapis arvensis seeds were extracted, 260/230 ratios greater than 1.8 were seen. RNA yields in excess of 10 µg per 100 mg seed suitable for RT-QPCR were obtained.
| Item Type: | Journal Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QK Botany Q Science > QP Physiology |
|||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
| Library of Congress Subject Headings (LCSH): | RNA, Arabidopsis thaliana, Brassica, Polysaccharides | |||||||||
| Journal or Publication Title: | Seed Science Research | |||||||||
| Publisher: | Cambridge University Press | |||||||||
| ISSN: | 0960-2585 | |||||||||
| Official Date: | December 2018 | |||||||||
| Dates: |
|
|||||||||
| Volume: | 28 | |||||||||
| Number: | 4 | |||||||||
| Page Range: | pp. 360-364 | |||||||||
| DOI: | 10.1017/S0960258518000296 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Reuse Statement (publisher, data, author rights): | This article has been accepted for publication in a revised form for publication in https://www.cambridge.org/core/journals/seed-science-research | |||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||
| Date of first compliant deposit: | 15 August 2018 | |||||||||
| Date of first compliant Open Access: | 15 August 2018 | |||||||||
| RIOXX Funder/Project Grant: |
|
|||||||||
| Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
