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SNP‐based genotyping and whole‐genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum , causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin
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Nakato, Gloria V., Studholme, David J., Blomme, Guy, Grant, Murray, Coutinho, Teresa A., Were, Evans M., Wicker, Emmanuel and Mahuku, George (2021) SNP‐based genotyping and whole‐genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum , causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin. Plant Pathology, 70 (3). pp. 534-543. doi:10.1111/ppa.13308 ISSN 1365-3059.
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WRAP-SNP‐based-genotyping-genome-sequencing-Xanthomonas-Ethiopian-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1324Kb) | Preview |
Official URL: https://doi.org/10.1111/ppa.13308
Abstract
For decades, Xanthomonas vasicola pv. musacearum (Xvm) has been an economically important bacterial pathogen on enset in Ethiopia. Since 2001, Xvm has also been responsible for significant losses to banana crops in several East and Central African countries, with devastating consequences for smallholder farmers. Understanding the genetic diversity within Xvm populations is essential for the smart design of transnationally reasoned, durable, and effective management practices. Previous studies have revealed limited genetic diversity in Xvm, with East African isolates from banana each falling into one of two closely related clades previously designated as sublineages SL 1 and SL 2, the former of which had also been detected on banana and enset in Ethiopia. Given the presumed origin of Xvm in Ethiopia, we hypothesized that both clades might be found in that country, along with additional genotypes not seen in Central and East African bananas. Genotyping of 97 isolates and whole‐genome sequencing of 15 isolates revealed not only the presence of SL 2 in Ethiopia, but additional diversity beyond SL 1 and SL 2 in four new clades. Moreover, SL 2 was detected in the Democratic Republic of Congo, where previously SL 1 was the only clade reported. These results demonstrate a greater range of genetic diversity among Xvm isolates than previously reported, especially in Ethiopia, and further support the hypothesis that the East/Central Africa xanthomonas wilt epidemic has been caused by a restricted set of genotypes drawn from a highly diverse pathogen pool in Ethiopia.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QK Botany S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Ensete , Genomics , Bananas -- Genetics, Bananas -- Diseases and pests , Bananas -- Diseases and pests -- Ethiopia, Wilt diseases | |||||||||||||||
| Journal or Publication Title: | Plant Pathology | |||||||||||||||
| Publisher: | Wiley | |||||||||||||||
| ISSN: | 1365-3059 | |||||||||||||||
| Official Date: | April 2021 | |||||||||||||||
| Dates: |
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| Volume: | 70 | |||||||||||||||
| Number: | 3 | |||||||||||||||
| Page Range: | pp. 534-543 | |||||||||||||||
| DOI: | 10.1111/ppa.13308 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Reuse Statement (publisher, data, author rights): | ** Article version: VoR ** From Crossref journal articles via Jisc Publications Router ** History: epub 27-11-2020; issued 27-11-2020. ** Licence for VoR version of this article starting on 27-11-2020: http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 9 December 2020 | |||||||||||||||
| Date of first compliant Open Access: | 11 December 2020 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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