ATCC14028s Study Group (Including: Achtman, Mark, Van den Broeck, Frederik, Cooper, Kerry K., Lemey, Philippe, Parker, Craig T. and Zhou, Zhemin). (2021) Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature. PLoS Genetics, 17 (9). e1009820. doi:10.1371/journal.pgen.1009820 ISSN 1553-7390.

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Abstract

Salmonella enterica serovar Typhimurium strain ATCC14028s is commercially available from multiple national type culture collections, and has been widely used since 1960 for quality control of growth media and experiments on fitness (“laboratory evolution”). ATCC14028s has been implicated in multiple cross-contaminations in the laboratory, and has also caused multiple laboratory infections and one known attempt at bioterrorism. According to hierarchical clustering of 3002 core gene sequences, ATCC14028s belongs to HierCC cluster HC20_373 in which most internal branch lengths are only one to three SNPs long. Many natural Typhimurium isolates from humans, domesticated animals and the environment also belong to HC20_373, and their core genomes are almost indistinguishable from those of laboratory strains. These natural isolates have infected humans in Ireland and Taiwan for decades, and are common in the British Isles as well as the Americas. The isolation history of some of the natural isolates confirms the conclusion that they do not represent recent contamination by the laboratory strain, and 10% carry plasmids or bacteriophages which have been acquired in nature by HGT from unrelated bacteria. We propose that ATCC14028s has repeatedly escaped from the laboratory environment into nature via laboratory accidents or infections, but the escaped micro-lineages have only a limited life span. As a result, there is a genetic gap separating HC20_373 from its closest natural relatives due to a divergence between them in the late 19th century followed by repeated extinction events of escaped HC20_373.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history Q Science > QR Microbiology
Divisions:	Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Salmonella enteritidis , Genomics, Gene mapping
Journal or Publication Title:	PLoS Genetics
Publisher:	Public Library of Science
ISSN:	1553-7390
Official Date:	27 September 2021
Dates:	Date Event 27 September 2021 Published 10 September 2021 Accepted
Volume:	17
Number:	9
Article Number:	e1009820
DOI:	10.1371/journal.pgen.1009820
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	17 September 2021
Date of first compliant Open Access:	13 October 2021
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID 202792/Z/ 16/Z Wellcome Trust http://dx.doi.org/10.13039/100010269 725422- ReservoirDOCS Horizon 2020 Framework Programme http://dx.doi.org/10.13039/100010661 2030-42000-055- 00D U.S. Department of Agriculture http://dx.doi.org/10.13039/100000199
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