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Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
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Bhandare, Amol M., van de Wiel, Joseph, Roberts, Reno, Braren, Inke, Huckstepp, Robert T. R. and Dale, Nicholas (2022) Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice. eLife, 11 . e70671. doi:10.7554/eLife.70671 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.70671
Abstract
Regulation of systemic PCO2 is a life-preserving homeostatic mechanism. In the medulla oblongata, the retrotrapezoid nucleus (RTN) and rostral medullary Raphe are proposed as CO2 chemosensory nuclei mediating adaptive respiratory changes. Hypercapnia also induces active expiration, an adaptive change thought to be controlled by the lateral parafacial region (pFL). Here we use GCaMP6 expression and head-mounted mini-microscopes to image Ca2+ activity in these nuclei in awake adult mice during hypercapnia. Activity in the pFL supports its role as a homogenous neuronal population that drives active expiration. Our data show that chemosensory responses in the RTN and Raphe differ in their temporal characteristics and sensitivity to CO2, raising the possibility these nuclei act in a coordinated way to generate adaptive ventilatory responses to hypercapnia. Our analysis revises the understanding of chemosensory control in awake adult mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QP Physiology | |||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | Brain stem, Respiratory organs, Chemoreceptors, Chemical senses, Animals -- Sense organs, Senses and sensation, Neurobiology | |||||||||
Journal or Publication Title: | eLife | |||||||||
Publisher: | eLife Sciences Publications Ltd. | |||||||||
ISSN: | 2050-084X | |||||||||
Official Date: | 27 October 2022 | |||||||||
Dates: |
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Volume: | 11 | |||||||||
Article Number: | e70671 | |||||||||
DOI: | 10.7554/eLife.70671 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 1 November 2022 | |||||||||
Date of first compliant Open Access: | 2 November 2022 | |||||||||
RIOXX Funder/Project Grant: |
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