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Novel D-glutamate catabolic pathway in marine Proteobacteria and halophilic archaea
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Yu, Yang, Wang, Peng, Cao, Hai-Yan, Teng, Zhao-Jie, Zhu, Yanping, Wang, Min, McMinn, Andrew, Chen, Yin, Xiang, Hua, Zhang, Yu-Zhong, Chen, Xiu-Lan and Zhang, Yu-Qiang (2023) Novel D-glutamate catabolic pathway in marine Proteobacteria and halophilic archaea. The ISME Journal, 17 . pp. 537-548. doi:10.1038/s41396-023-01364-6 ISSN 1751-7362.
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Official URL: https://doi.org/10.1038/s41396-023-01364-6
Abstract
D-glutamate (D-Glu) is an essential component of bacterial peptidoglycans, representing an important, yet overlooked, pool of organic matter in global oceans. However, little is known on D-Glu catabolism by marine microorganisms. Here, a novel catabolic pathway for D-Glu was identified using the marine bacterium Pseudoalteromonas sp. CF6-2 as the model. Two novel enzymes (DgcN, DgcA), together with a transcriptional regulator DgcR, are crucial for D-Glu catabolism in strain CF6-2. Genetic and biochemical data confirm that DgcN is a N-acetyltransferase which catalyzes the formation of N-acetyl-D-Glu from D-Glu. DgcA is a racemase that converts N-acetyl-D-Glu to N-acetyl-L-Glu, which is further hydrolyzed to L-Glu. DgcR positively regulates the transcription of dgcN and dgcA. Structural and biochemical analyses suggested that DgcN and its homologs, which use D-Glu as the acyl receptor, represent a new group of the general control non-repressible 5 (GCN5)-related N-acetyltransferases (GNAT) superfamily. DgcA and DgcN occur widely in marine bacteria (particularly Rhodobacterales) and halophilic archaea (Halobacteria) and are abundant in marine and hypersaline metagenome datasets. Thus, this study reveals a novel D-Glu catabolic pathway in ecologically important marine bacteria and halophilic archaea and helps better understand the catabolism and recycling of D-Glu in these ecosystems.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Glutamic acid , Peptidoglycans, Marine microbial ecology , Marine bacteria, Microbial metabolism | |||||||||||||||||||||
Journal or Publication Title: | The ISME Journal | |||||||||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||||||||
ISSN: | 1751-7362 | |||||||||||||||||||||
Official Date: | April 2023 | |||||||||||||||||||||
Dates: |
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Volume: | 17 | |||||||||||||||||||||
Page Range: | pp. 537-548 | |||||||||||||||||||||
DOI: | 10.1038/s41396-023-01364-6 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||
Date of first compliant deposit: | 20 June 2023 | |||||||||||||||||||||
Date of first compliant Open Access: | 21 June 2023 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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