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Highly sensitive and label-free detection of biotin using liquid crystal-based optofluidic biosensor
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Wang, Haonan, Xu, Tianhua, Wang, Ziyihui, Liu, Yize, Chen, Huaixu, Jiang, Junfeng and Liu, Tiegen (2023) Highly sensitive and label-free detection of biotin using liquid crystal-based optofluidic biosensor. Biomedical Optics Express, 14 (7). pp. 3763-3774. doi:10.1364/BOE.494783 ISSN 2156-7085.
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Official URL: https://doi.org/10.1364/BOE.494783
Abstract
A liquid crystal (LC)-based optofluidic whispering gallery mode (WGM) resonator has been applied as a biosensor to detect biotin. Immobilized streptavidin (SA) act as protein molecules and specifically bind to biotin through strong non-covalent interaction, which can interfere with the orientation of LCs by decreasing the vertical anchoring force of the alignment layer in which the WGM spectral wavelength shift is monitored as a sensing parameter. Due to the double magnification of the LC molecular orientation transition and the resonance of the WGM, the detection limit for SA can reach 1.25 fM (4.7 × 10−13 g/ml). The measurable concentration of biotin and the wavelength shift of the WGM spectrum have an excellent linearity in the range of 0 to 0.1 pg/ml, which can achieve ultra-low detection limit (0.4 fM), i.e., seven orders of magnitude improvement over conventional polarized optical microscope (POM) method. The proposed optofluidic biosensor is highly reproducible and can be used as an ultrasensitive real-time monitoring biosensor, which will open the door for applications to other receptor and ligand models.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QP Physiology Q Science > QR Microbiology T Technology > TJ Mechanical engineering and machinery T Technology > TS Manufactures |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Biotin, Liquid crystal devices, Optofluidics , Microresonators (Optoelectronics), Optical detectors, Biosensors , Streptavidin | |||||||||||||||
Journal or Publication Title: | Biomedical Optics Express | |||||||||||||||
Publisher: | Optical Society of America | |||||||||||||||
ISSN: | 2156-7085 | |||||||||||||||
Official Date: | 1 July 2023 | |||||||||||||||
Dates: |
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Volume: | 14 | |||||||||||||||
Number: | 7 | |||||||||||||||
Page Range: | pp. 3763-3774 | |||||||||||||||
DOI: | 10.1364/BOE.494783 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Copyright Holders: | © 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement | |||||||||||||||
Date of first compliant deposit: | 21 June 2023 | |||||||||||||||
Date of first compliant Open Access: | 27 July 2023 | |||||||||||||||
RIOXX Funder/Project Grant: |
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