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Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation
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Gao, Yanan, Bissoyi, Akalabya, Kinney, Nina L. H., Whale, Thomas F., Guo, Qiongyu and Gibson, Matthew I. (2023) Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation. Chemical Communications, 59 (59). pp. 9086-9089. doi:10.1039/D3CC02252H ISSN 1359-7345.
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Official URL: http://dx.doi.org/10.1039/D3CC02252H
Abstract
Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids. This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry | |||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Proline, Nucleation, Ice, Cell aggregation, Cryopreservation of organs, tissues, etc. | |||||||||||||||||||||
Journal or Publication Title: | Chemical Communications | |||||||||||||||||||||
Publisher: | Royal Society of Chemistry | |||||||||||||||||||||
ISSN: | 1359-7345 | |||||||||||||||||||||
Official Date: | 28 July 2023 | |||||||||||||||||||||
Dates: |
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Volume: | 59 | |||||||||||||||||||||
Number: | 59 | |||||||||||||||||||||
Page Range: | pp. 9086-9089 | |||||||||||||||||||||
DOI: | 10.1039/D3CC02252H | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 6 July 2023 | |||||||||||||||||||||
Date of first compliant Open Access: | 7 July 2023 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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